Apex Bioscience, Inc.

University of Texas System

Duke University

Other

Strohtech, Inc.

Joseph De Angelo

Joseph Bonaventura

Robert G Kilbourn

Nalini M Motwani

Wajeeh Bajwa

The invention is directed to a method for the prophylaxis or treatment of an animal for deleterious physiological effects such as systemic hypotension caused by nitric oxide production induced by a biological response modifier. Examples of such biological response modifiers include but are not limited to a cytokine and an endotoxin. The invention is also directed to a method for the treatment of septic shock.

Inhibition of nitric oxide-mediated hypotension and septic shock with iron-containing hemoprotein

The invention is directed to a substantially pure mammalian globin chain or heme-binding fragment thereof. The invention is further directed to recombinant DNA vectors capable of expressing at least one globin chain or substantially homologous variant thereof in yeast. The invention also relates to methods for expressing at least one globin chain or substantially homologous variant thereof in yeast. Expressed alpha-like globin and beta-like globin chains or variants thereof may be combined with a source of heme to produce hemoglobin or a substantially homologous variant thereof. Additionally, expressed gamma-globin chains may be combined with a source of heme to produce hemoglobin or a substantially homologous variant thereof. The invention also relates to methods for expressing hemoglobin or variants thereof in yeast where the heme is produced by the yeast and ligated to globins to form hemoglobin in vivo. The hemoglobin produced by the methods of the present invention may be used in applications requiring a physiological oxygen carrier such as in blood substitute solutions and as in plasma expanders or in applications requiring a physiological oxygen carrier.

Expression of recombinant hemoglobin and hemoglobin variants in yeast

Prevention or inhibition of pathogenic syndromes caused by nitric oxide

The invention is directed to a method for the prophylaxis or treatment of an animal for deleterious physiological effects such as systemic hypotension caused by nitric oxide production induced by a biological response modifier. Examples of such biological response modifiers include but are not limited to a cytokine and an endotoxin. The invention is also direction to a method for the treatment of septic shock.

Use of iron hemoprotein for treating pathogenic syndrome caused by

Treatment of septic shock with iron-containing hemoprotein

Inhibition of nitric oxide-mediated hypotension and septic shock with

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Hamtramck, MI, United States of America

Joseph De Angelo