Corning Incorporated

Joseph Christopher Wall

David A Kenney

Gregory Roger Martin

Allison Jean Tanner

George F Lyman

Anthony M Labriola

Kathy Marie Youngbear

Todd M Upton

Henry J Cattadoras

Nikolaos Pantelis Kladias

William Joseph Lacey

Scott Matthew Bennett

Henry Joseph Cattadoris

Aravind Raghavan Rammohan

John A Ryan

Connie Marie Head

Thomas Jay Beams

Jin Wang

Shang-Pin Kwei

Laurence M Vaughan

Alan Craig Wright

David Alan Kenney

Michael Patrick Keenan

A 5-liter bioreactor vessel enables a substantial increase in the volume of biological media that can be cultivated in the vessel compared to conventional designs. Moreover, when agitated at 1.5&#xd7; the shaking frequency of a conventional 3-liter bioreactor (i.e., 90 rpm versus 60 rpm), the 5-liter vessel achieves a 19% increase in cell aeration without exceeding the maximum shear stress limit for cell viability. The 5-liter vessel optionally includes a plurality of internal baffles configured to disrupt the liquid vortex and reduce the maximum shear stress transferred to biomedia contained within the vessel.

Vessel for growth of biological entities

A cell culture apparatus includes cell culture units having cell culture chambers, each with at least one manifold. The manifold connects the cell culture unit to a fluid flow channel. The manifold has a dam which allows liquid to pool in the manifold and allows for the creation of an airspace in the manifold, which reduces hydrostatic pressure inside the apparatus and enables the stacking of multiple cell culture units. Embodiments include cascading manifolds, manifolds having reservoirs, and manifolds having valves.

Cell culture system with manifold

The disclosure provides a cell culture apparatus having multiple stacked cell culture units each having multiple cell culture chambers. The multiple cell culture units are connected by a manifold which connects the cell culture units and provides a mechanism for efficiently filling the multiple cell culture chambers.

Arched cell culture flask

Cell culture flask

A device for trapping extraneous gas or bubbles is disclosed. The device is defined as having a gas collection area in a tapered neck portion such that when used as a cell culturing apparatus, extraneous gas or bubbles are trapped and isolated from the cell growth areas where cells or media reside.

Device for trapping extraneous gas

A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus includes a unitary flask body including a rigid upper and lower surface, connected by side walls. The cell growth apparatus comprises multiple cell growth chambers stacked in vertical alignment and orientation within the unitary flask body. The stacked chambers are held in position by unitary connecting columns that run through each cell growth chamber and terminate at the rigid upper and lower surfaces of the apparatus. The cell growth chambers are separated by tracheal spaces that allow air from the external environment to contact the cell growth surface of each individual cell growth chamber.

Multilayer cell culture vessels

A device for minimizing the formation of bubbles or foam in cell culture is disclosed. The device has a manifold which directs the inflow of cells and cell culture media into a cell culture vessel so as to allow for displaced air or gas to vent from the cell culture vessel without mixing with the incoming cell culture media, thereby preventing the mixing of air and cell culture media and minimizing the formation of bubbles or foam inside the cell culture vessel.

Device and method for reducing bubble formation in cell culture

Filter apparatus

A stackable flask for the culturing of cells is disclosed. The cell culture chamber is defined by a top plate and a rigid bottom tray of substantially rectangular shape connected by side and end walls, the body of the flask has imparted therein a gas permeable membrane that will allow the free flow of gases between the cell culture chamber and the external environment. The flask body also includes a sealed septum that will allow access to the cell growth chamber by means of a needle or cannula. The size of the flask and location of an optional neck and cap section allows for flask manipulation by standard automated assay equipment, making the flask ideal for high throughput applications.

Cell cultivating flask

A microorganism culture plate and methods for its fabrication and use is provided. The culture plate comprises a splash-guard that attaches to an upper rim of the plate. The splash-guard forms a removable, hermetic seal with the rim to prevent liquid media or other fluids from spilling or leaking out of the plate. The splash-guard as defined by a frame of film, according to an embodiment, has an aperture situated over a volume of the culture plate.

Removable splash guards for culture plates

The present invention includes a cell cultivating flask and a method for growing cells within the cell cultivating flask which is configured such that a user can directly access the individual trays or cell growing areas within the cell cultivating flask. Basically, the cell cultivating flask includes a cover located on top of an intermediate tray which is located on top of a bottom tray. The intermediate tray and bottom tray each have a bottom plate and side walls that define a cell growth area. And, the cover and intermediate tray each have a neck with an opening formed therein which enables a user to directly add or remove a cell cultivating media to or from each of the cell growth areas in the intermediate tray and the bottom tray. The cell cultivating flask may have more than one intermediate tray.

Cell cultivating flask and method for using the cell cultivating flask

A reusable pipette-tip holder that doubles the density of the number of pipette tips able to be stored within a single container, while conserving the total amount of material, packaging and storage space. The invention creates a rigid box by the joining, bottom to bottom, of two pipette-racks each having a standard matrix of pipette-receiving holes. The new design permits pipettes to be inserted from both the top and bottom of the box, because the centerline spacing of the rows of pipette-receiving holes on the top and bottom surfaces is offset with respect to each other. Rows of pipette tips from one side of the container nest between the free space in adjacent rows of tips inserted from the other side of the container.

Growing community of inventors

Southborough, MA, United States of America

Joseph Christopher Wall