Danisco US Inc.

The Goodyear & Tire Rubber Company

Genencor International, Inc.

Dow Corning Corporation

United States of America As Represented by the Secretary of the Air Force

Canji Inc.

Joseph Charles McAuliffe

Gregory Marshall Whited

Karl John Sanford

Gopal K Chotani

Marguerite A Cervin

Caroline M Peres

Frank James Feher

Andrei Miasnikov

Richard La Duca

Fernando Valle

Aaron Scott Puhala

Timothy A Sabo

Derek H Wells

Michael C Miller

Wyatt Charles Smith

Anthony R Calabria

Stephan Rodewald

Rachel E Muir

Erin L Spring

Huaming Wang

Walter Weyler

Risha Lindig Bond

Carol Marie Fioresi

Dmitrii V Vaviline

Sergey Paramonov

Ilana S Aldor

Richard Ray Bott

James T Kellis, Jr

Eric Jude Joffre

Bradley R Kelemen

Alex Toftgaard Nielsen

Tang Hong Wong

Christopher Lee Rife

Rasmus B Jensen

Donald E Ward, Ii

Samira Rathnayake

Michael C Jewett

John Kaluen Kan

Thomas F McCall

Lawrence J Pickert

Christopher D Ploetz

John C Saam

Rajesh R Naik

Robert James Connor

Richard A Vaia

Michael Stephen Starch

William Albert Cuevas

Thomas Howard Lane

Kurt Friedrich Brandstadt

Xavier Thomas

Virginia Kay O'Neil

Csilla Kollar

David Clayton Gantner

Lorry Deklippel

Christel Simon

Lawrence F Drummy

Erin L Webster

Benoit Henault

Serge Firmin Alain Creutz

Fernando Vazquez Carrillo

Michael Costello

The invention features methods for producing isoprene from cultured cells wherein the cells in the stationary phase. The invention also provides compositions that include these cultured cells and/or increased amount of isoprene. The invention also provides for systems that include a non-flammable concentration of isoprene in the gas phase. Additionally, the invention provides isoprene compositions, such as compositions with increased amount of isoprene or increased purity.

Compositions and methods for producing isoprene free of c5 hydrocarbons under decoupling conditions and/or safe operating ranges

The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.

Compositions and methods for producing isoprene

Compositions and methods for producing isoprene free of C5 hydrocarbons under decoupling conditions and/or safe operating ranges

The invention features methods producing isoprene and a co-product, such as ethanol, 1,3-propanediol, or hydrogen from cultured cells. The invention also provides compositions that include these cultured cells. The invention provides compositions comprising isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen. Additionally, the invention provides methods of co-producing isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen by culturing cells under conditions suitable for co-production of isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen.

Methods of producing isoprene and a co-product

Methods and apparatus for the purification of isoprene, such as the purification of a bioisoprene composition from fermentor off-gas. The apparatus includes two columns that process the fermentor off-gas, which includes isoprene and various impurities. A solvent is added to the off-gas in the first column, and the isoprene is stripped from the solvent in the second column. Also provided is a downstream further purification process. Also provided are the resulting purified isoprene compositions.

Purification of isoprene from renewable resources

This invention relates to recombinant microorganisms capable of producing isoprene and isoprene production with the use of such recombinant microorganism with good efficiency. In this invention, functional activity of the ispA gene is altered to reduce the production of isoprenoid molecules in recombinant cells engineered to produce isoprene or in cells otherwise susceptible to isoprenoid accumulation during fermentation. This decreased ispA gene functional activity enables enhanced synthesis of isoprene in a host microorganism.

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It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has &#x3b4;C value of greater than &#x2212;22%.

Polymers of isoprene from renewable resources

The invention features methods for producing isoprene from cultured cells wherein the cells in the stationary phase. The invention also provides compositions that include these cultured cells and/or increased amount of isoprene. The invention also provides for systems that include a nonflammable concentration of isoprene in the gas phase. Additionally, the invention provides isoprene compositions, such as compositions with increased amount of isoprene or increased purity.

The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells. The invention provides isoprene compositions, such as compositions with increased amount of isoprene or increased purity. Additionally, the invention provides methods of producing isoprene by culturing cells under conditions suitable for isoprene production while maintaining cell viability and/or metabolic activity.

Systems using cell culture for production of isoprene

Enhanced production of isoprene using host cells having decreased ispA activity

The present invention provides methods for producing derivatives from cultured cells. In addition, the present invention provides methods for conversion of prenyl derivatives, obtained from biological or petrochemical sources, to isoprene by employing chemical or biological catalysts. The present invention also provides compositions that include the cultured cells or isoprene or prenyl derivatives produced there from.

Conversion of prenyl derivatives to isoprene

It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has &#x3b4;C value of greater than &#x2212;22&#x2030;.

Polymers of isropene from renewable resources

Methods, compositions and systems using isoprene from a bioisoprene composition derived from renewable carbon for production of a variety of hydrocarbon fuels, fuel additives, and additives for fine chemistry and other uses is described.

Fuel compositions comprising isoprene derivatives

Growing community of inventors

Sunnyvale, CA, United States of America

Joseph Charles McAuliffe