Integrated Dna Technologies, Inc.

University of Iowa Research Foundation

Walder, Joseph Alan

Behlke, Mark Aaron

Owczarzy, Richard

Lennox, Kimberly Ann

Jacobi, Ashley Mae

Joseph Alan Walder

Mark Aaron Behlke

Andrei Laikhter

Yawfui Yong

Scott Rose

Richard Owczarzy

Mikhail Podyminogin

Joseph R Dobosy

Jeffrey A Manthey

Roxanne Y Walder

Kevin William Roberts

Mirna Jarosz

Caifu Chen

Lingyan Huang

Shawn Allen

John M Dagle

Zachary Zwirko

Yu Zheng

Yong You

Eric Jeffrey Devor

Paul S Eder

William E Martin, Iii

Susan M Rupp

Derek M Thomas

Michael R Marvin

Kim Lennox

Kristin Beltz

Ashley Mae Jacobi

Joesph Dobosy

Kimberly Ann Lennox

Bernardo Moreira

Yawful Yong

Shale Anthony James

Joseph Dobosy

Scott D Rose

Yon You

The invention pertains to construction of next-generation DNA sequencing (NGS) libraries for whole genome sequencing, targeted resequencing, sequencing-based screening assays, metagenomics, or any other application requiring sample preparation for NGS.

Construction of next generation sequencing (NGS) libraries using competitive strand displacement

Disclosed herein are nucleic acid-based data storage systems and nucleic acid data storage constructs comprising reusable nucleic acid sequences, each representing information carried by a single bit (and, in some embodiments, one or more adjacent bits) within a bit string, and each furthermore representing the position of the single bit within the bit string. Also described are methods for storing data in the nucleic acid-based data storage systems and nucleic acid data storage constructs of the disclosure.

DNA data storage using reusable nucleic acids

This invention relates to mutant DNA polymerases having an enhanced template discrimination activity compared with the corresponding unmodified DNA polymerase counterparts, wherein the amino acid sequence of the mutant DNA polymerase includes at least one substitution at residue positions structurally and functionally homologous or orthologous positions 783 or 784 of an unmodified Taq DNA polymerase.

DNA polymerase mutants having enhanced template discrimination activity

The invention describes composition and methods of use for novel hairpin blocked-cleavable primers. In one embodiment unblocking occurs through action of RNase H2. The method improves the specificity of PCR and reduces primer dimer events, enabling higher level multiplex reactions. Additionally, the invention protects RNA-containing primers from attack by single-strand RNases.

Cleavable hairpin primers

Methods and compositions are provided for improving specificity during amplification of a target DNA sequence. The methods and compositions rely upon the use of an RNase H enzyme, a polymerase, and RNase H enzyme-sensitive, blocked-cleavable oligonucleotide primers in the amplification reactions, wherein the reaction mixtures include either an optimized final concentration of a divalent metal salt comprising 2.0 mM or less of free Mgcation and/or an optimized final concentration of a non-ionic detergent comprising at least about 0.001% polyethylene glycol hexadecyl ether.

RNase H-based assays utilizing modified RNA monomers

The invention pertains to modifications for antisense oligonucleotides, wherein the modifications are used to improve stability and provide protection from nuclease degradation. The modifications could also be incorporated into double-stranded nucleic acids, such as synthetic siRNAs and miRNAs.

Modifications for antisense compounds

The invention is directed to an in vitro method for joining a first set of double-stranded (ds) DNA molecules. Small molecules acting as chaperone agents are identified that promote efficient and rapid assembly (that is, joining) of overlapping double-stranded DNA fragments.

Efficient and rapid method for assembling and cloning double-stranded DNA fragments

The present invention pertains to novel oligonucleotide compounds for use in various biological assays, such as nucleic acid amplification, ligation and sequencing reactions. The novel oligonucleotides comprise a ribonucleic acid domain and a blocking group at or near the 3' end of the oligonucleotide. These compounds offer an added level of specificity previously unseen. Methods for performing nucleic acid amplification, ligation and sequencing are also provided. Additionally, kits containing the oligonucleotides are also disclosed herein.

Disclosed is a group of azo quencher compositions useful as fluorescence quenchers having the general structure of formula 1, methods of making or using the compositions, and kits comprising the composition. 

Fluorescence quenching azo dyes, their methods of preparation and use

The present invention provides methods of cleaving a nucleic acid strand to initiate, assist, monitor or perform biological assays.

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Chicago, IL, United States of America

Joseph Alan Walder