Cargill Inc.

United States of America, As Represented by the Secretary of Agriculture

Wisconsin Alumni Research Foundation

Levadura Biotechnology, Inc.

Jose Miguel Laplaza

Paula M Hicks

Sherry R Kollmann

Sara C McFarlan

Fernando A Sanchez-Riera

Christopher Solheid

Mervyn L De Souza

Steven John Gort

Thomas W Jeffries

Brian J Brazeau

David Paul Weiner

Peter Luginbuhl

Wei Niu

James C Anderson

Analia Bueno

Erin Kathleen Marasco

Joslin M Cuenca

Alexander Hutagalung

Mervyn L deSouza

Joshua Lundorff

Haiying Ni

Strains of yeasts are provided containing the genes for the production of cannabinoids from fatty acids. The enzymes that mediate cannabinoid production are localized to the cytosol, peroxisome or different compartments within the secretory pathway (e.g., endoplasmic reticulum, Golgi, vacuole) to ensure efficient production. The engineered microorganisms produce cannabinoids in a controlled fermentation process.

Production of cannabinoids in modified yeast using a fatty acid feedstock

Monatin and certain stereoisomers of monatin, such as R,R monatin and S,R monatin, as well as salts thereof, are produced using polypeptides and biosynthetic pathways. These polypeptides and biosynthetic pathways are also useful in the production of R-2-hydroxy-2-(indoly-3-ylmethyl)-4-keto glutaric acid, an intermediate that is formed in certain monatin synthesis pathways, including some biosynthetic pathways.

Polypeptides and biosynthetic pathways for the production of stereoisomers of monatin and their precursors

The invention provides for aminotransferase and oxidoreductase polypeptides and nucleic acids encoding such polypeptides. Also provided are methods of using such aminotransferase and oxidoreductase nucleic acids and polypeptides.

Aminotransferase and oxidoreductase nucleic acids and polypeptides and methods of using

Products and methods for the in vivo production of monatin sweetener are provided. The products include microorganisms that are genetically modified to secrete or to improve secretion of monatin; microorganisms that are genetically modified to produce monatin; and microorganisms that are genetically modified to both secrete or improve secretion of monatin and produce monatin. The methods include producing monatin in such genetically engineered microorganisms.

Products and methods for in vivo secretion of monatin

Disclosed are methods for obtaining expression of polypeptides in organisms employing alternative codon systems, and polynucleotides for use therein.

Yeast transformation system

Disclosed are xylose-fermenting recombinant yeast strains expressing xylose reductase, xylitol dehydrogenase, and xylulokinase and having reduced expression of PHO13 or a PHO13 ortholog, as well as methods of fermenting xylose to obtain ethanol using the recombinant yeast strains.

Xylose-fermenting recombinant yeast strains

Growing community of inventors

Plymouth, MN, United States of America

Jose Miguel Laplaza