Universidad De Valladolid

Jose Miguel Ferreras Rodriguez

Francisco Javier Arias Vallejo

Raquel Munoz Martinez

Pilar Jimenez Lopez

Tomas Girbes Juan

Rosario Iglesias Alvarez

Ma Angeles Rojo Rodriguez

Lucia Citores Gonzalez

Fernando Martinez De Benito

The invention discloses ribosome inactivating proteins (RIPs) with two chains, which arc not toxic in the extra-cellular environment, of plant origin, and capable of interacting with ribonucleic acid and causing the inhibition of the biosynthesis of proteins in cellular systems, said proteins being comprised of two chains A and B, chain A having a N-glucosidase activity of the ribosomal ribonucleic acid, and chain B having a lectine activity, both chains being joined by disulphide bridges. Among the proteins, Nigrine b isolated from bark of Sambucus nigra L., basic Nigrine l isolated from the leaves of Sambucus nigra L., Ebuline 1 isolated from leaves of Sambucus ebulus L. and Racenosine b isolated from the bark of Sambucus racemosa L. are mentioned. The proteins find application as inactivators of the rebonucleic acid and as inhibitors of the protein synthesis.

Non-toxic ribosome inactivating proteins (RIPs) with two chains, process

Growing community of inventors

Valladolid, Spain

Jose Miguel Ferreras Rodriguez