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Jose Luis Pelegrino Martinez De La Cotera

Hilda Elisa Garay Perez

Orlando Luis Pardo Lazo

Guadalupe Guzman Tirado

Susana Vazquez Ramudo

Osvaldo Reyes Acosta

Ana Beatriz Perez Diaz

Maritza Pupo Antunez

Glay Chinea Santiago

Rosmari Rodriguez Roche

Gabriel Padron Palomares

Luis Morier Diaz

Maylin Alvarez Vera

Omaida Perez Insuita

The present invention relates to five synthetic peptides of pre-M/M protein of Dengue-2 virus, corresponding to amino acid sequences 3-31, 45-67, 57-92, 69-93 and 103-124. The anti-peptide immune response was evaluated in mice. Recombinant fusion proteins were also obtained, including regions of pre-M/M protein. The presence of B cell epitopes in both mice and humans was demonstrated in the pre-M/M protein peptides. Peptides 3-31 and 103-124 elicited neutralizing antibodies against the four serotypes of Dengue virus. Virus-specific proliferative responses were demonstrated in mice immunized with non-conjugated peptides 3-31 and 57-92. Mice immunized with conjugated peptides 3-31, 57-92, and 69-93 were protected when they were challenged with Dengue-2 virus. Thus, the presence of sequential epitopes in Pre-M/M protein of Dengue-2 virus was demonstrated, as well as their relevance in the immune response against this flavivirus.

Pre-M/M epitopes of dengue virus, synthetic peptides, chimeric proteins and their use

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Habana, Cuba

Jose Luis Pelegrino Martinez De La Cotera