Erasmus University Medical Center Rotterdam

Universidad De Salamanca

Cytognos, S.l.

Uniersidad De Salamanca

Academisch Ziekenhuis Leiden (h.o.d.n. Lumc)

University of Salamanca

Stichting Euroflow

José Alberto Orfao De Matos Correia E Vale

Jacobus Johannes Maria Van Dongen

Vincent Henricus Johannes Van Der Velden

Julia Maria Almeida Parra

Sebastian Böttcher

Paulo Jorge Monteiro Da Silva Lucio

Carlos Eduardo Pedreira

Juan Alejandro Flores-Montero

Tomasz Szczepanski

Vahid Asnafi

Ludovic Bernard Simon Lhermitte

Ruth Mary De Tute

Andrew Craig Rawstron

Ester Mejstríková

Jacobus Johannes Van Dongen

Menno Cornelis Van Zelm

Vincent Henricus Van Der Velden

Sebastian Bottcher

Marta Martín Ayuso

Anne-Kathrin Kienzler

Elena Blanco Álvarez

Eduardo López-Granados

Marcela Vlková

Tomás Kalina

Mirjam Van Der Burg

Matthias Ritgen

Tomasz Szczepański

Juan Alejandro Flores Montero

Anthonie Willem Langerak

Elaine Sobral Da Costa

Martin Pérez-Andrés

Martín Pérez-andrés

Marta Marín Ayuso

Juan Bernardo Hernández Herrero

Rafael Fluxá Rodriguez

Ester MEJSTRÍKOVÁ

Rafael FluxÁ RODRÍGUEZ

Marta Marin Ayuso

Ludovic Bernard Lhermitte

Cristiane De Sa Ferreira Facio

Vitor Dessanti Botafogo Gonçalves

The invention relates to the field of flow cytometry and more particularly to a panel of antibody reagents conjugated to fluorescent compounds. Provided are reagent compositions, comprising at least eight distinct fluorochrome-conjugated antibodies comprising a set of at least there identification antibodies for the identification of a leukocyte population of interest and at least four characterization antibodies for further characterization and/or classification of said leukocyte population. Also provided are kits and methods related to the reagent compositions.

Methods, reagents and kits for flow cytometric immunophenotyping

This invention relates to the field of primary immunodeficiencies (PID), more specifically to means and method for the diagnosis of PID of the lymphoid system. Provided are unique reagent compositions for the flow cytometric immunophenotyping of leukocytes comprising fluorochrome-conjugated antibodies directed against various specific combinations of markers. Also provided are kits comprising the reagent compositions, and methods using the same.

Reagents, methods and kits for diagnosing primary immunodeficiencies

The invention relates to the field of minimal residual disease (MRD) diagnostics, which is progressively more applied for the evaluation of treatment effectiveness in patients with a hematological malignancy, such as B-cell precursor acute lymphoblastic leukemia (BCP-ALL), B-cell chronic lymphocytic leukemia (B-CLL), and multiple myeloma (MM). Provided are unique reagent compositions with carefully selected and thoroughly tested combinations of antibodies, for &#x2265;8-color flow cytometric stainings as well as for 10-color and 12-color flow cyometric stainings, which can reach sensitivities of at least 10, even down to 10. Also provided are diagnostic kits and methods for detecting MRD.

Methods, reagents and kits for detecting minimal residual disease

The invention relates to the field of medical diagnostics. Provided are methods and kits for determining the health status of a subject, for early detection of tissue damage, for early diagnosis and monitoring of a disease, and/or for evaluation of treatment effectiveness in a subject using circulating tissue macrophages (CTM) as a mirror of disrupted tissue homeostasis and disease.

Methods and means for monitoring disruption of tissue homeostasis in the total body

The invention relates to a computer-implemented method for classifying events present in a sample, wherein each event is characterized by a multidimensional set of parameters obtained by means of hardware and/or software, wherein the values of the parameters associated with each event define the position coordinates of said event in a multidimensional space. Said method comprises the following stages: a) clustering the events in groups, b) checking if within each formed group there is a connection between events exceeding a maximum distance threshold, c) calculating the affinity between each pair of previously generated sample groups, d) comparing each sample group with at least one reference group stored in at least one database, e) classifying the sample groups based on the comparisons with the reference groups.

Method of digital information classification

The invention relates to the field of flow cytometry and more particularly to a panel of antibody reagents conjugated to fluorescent compounds. Provided are reagent compositions, comprising at least eight distinct fluorochrome-conjugated antibodies comprising a set of at least three identification antibodies for the identification of a leukocyte population of interest and at least four characterization antibodies for further characterization and/or classification of said leukocyte population. Also provided are kits and methods related to the reagent compositions.

The invention relates to the field of medical diagnostics. In particular, it relates method and kits for identification and classification of IgE-related diseases, e.g. Type I hypersensitivity, as well as for monitoring of treatment efficacy, for instance anti-IgE therapy. Provided is a multi-color flow cytometric method for analyzing memory B cell and plasma cell subsets in a biological sample, comprising staining the sample with a panel of fluorochrome-conjugated antibodies comprising antibodies against IgM, IgA, IgG, IgD and IgE; an antibody against a B cell marker and an antibody against the CD38 antigen; subjecting the sample to flow cytometry and gating for lymphocytes based on forward scatter and side scatter pattern; gating the lymphocytes for expression of the B cell specific marker and CD38 to discriminate between CD38memory B cells and CD38plasma cells; and quantitating the IgE+ cells within the memory B cell population and/or the plasma cell population by the negative selection of cells expressing IgM, IgA, IgG and/or IgD and the positive selection of IgE expressing cells.

Methods and kits for detecting IgE-expressing B cells

This invention relates to methods to detect the presence of cancer infiltration of the Central Nervous System (CNS) based on the detection of soluble proteins, preferably, in cerebrospinal fluid samples and vitreous fluid. The invention also relates to kits to perform the methods of the invention.

Methods and kits for the detection of cancer infiltration of the central nervous system

A single measurement can quantify phosphatidyl-inositol glycan complementation class A (PIG-A) associated proteins in red cells, platelets and the major leukocyte subsets present in blood (lymphocytes, monocytes, neutrophils and eosinophils). The single measurement relies on flow cytometry to simultaneously evaluate intensity of fluorochrome emissions and light scattering properties.

Simultaneous quantification of PIG-A associated proteins in red cells, platelets and leukocyte subsets using a single measurement

A method that generates new flow cytometry data files with a potentially infinite number of dimensions is described. First two or more separate flow cytometry data files containing information about events measured in different aliquots from the same sample are fused. Then, the magnitude of the values and a corresponding uncertainty measure of the differing variables between one or more of the original data files which have been fused are estimated, for each individual event contained in the fused data file, for which that variable was not directly measured in the flow cytometer. The new data file containing both data actually measured in the flow cytometer for each event of the individual original data files fused and the estimated data for those parameters not measured in a group of events from one or more of the original data files are reconstructed multidimensionally.

Method for generating new flow cytometry data files containing an infinite number of dimensions based on data estimation

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Salamanca, Spain

José Alberto Orfao De Matos Correia E Vale