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Degussa - Huls Ag

Jörn Kalinowski

Klaus Huthmacher

Alfred Pühler

Walter Pfefferle

Alfred Puhler

Georg Thierbach

Brigitte Bathe

Bettina Möckel

Nicole Dusch

Patrick Schwientek

Hermann Wehlmann

Winfried Rosen

Udo Wehmeier

Klaus Selber

Andreas Tauch

Mike Farwick

Achim Marx

Michael Binder

Michael Hartmann

Andreas Schafer

Andreas Klein

Frank Elischweski

Jurgen Dohmen

Thomas Hermann

Mechthild Rieping

Bettina M&ouml;ckel

Alfred P&uuml;hler

Anke Weissenborn

Bernhard Weingärtner

Friedrich Kassing

Marcus Persicke

Susanne Schneiker-bekel

Lena Schaffert

Bettina Hädrich

Stephan Berens

Bernhard Weingaertner

Oliver Kirchner

Christian Rückert

Alfred Prof Pühler

Daniel Rey

The present invention relates to Actinomycetales strains for the improved formation of acarbose. Provided are Actinomycetales strains which are engineered to overexpress dTDP-D-glucose-4,6-dehydratase (AcbB) and/or uridyltransferase (GtaB). Also provided are Actinomycetales strains which are engineered to have a reduced or absent expression of the small carbohydrate binding protein (Cgt) and/or a reduced or absent expression of genes which are essential for carotenoid synthesis. Also provided are tools, methods and means to generate these strains.

Methods for the improved formation of acarbose

The present invention describes the DNA-sequence of the wild type genome as well as all genetic modifications which were introduced into the wild type&#x2014;and further developed strains, based thereon. Thereby the first genotypic characterization of the developed strains, including the latest production strain, has been accomplished, accounting for the major part of the invention. Furthermore, on the basis of the determined DNA-sequences, potential genes were identified and account, combined with their functional annotation, for another part of the invention. In particular, the gene- and DNA-sequences, as well as protein-sequences derived there out, contribute to the invention which were affected by mutagenic modifications throughout the strain development process, potentially contributing to the increased production yield.

Genomics of actinoplanes utahensis

The present invention is directed to an innate DNA sequence within the complete genome sequence ofsp. SE50/110 which resembles the structure of an actinomycete integrative and conjugative element (AICE). Related AICEs were used for establishing genetic manipulation tools for other bacteria in the past. In this document, we describe the unique features of the specific AICE found insp. SE50/110 which are clearly distinct from any other known AICE as a whole, but share minor parts with varying sequence similarity with other characterized AICEs from other species.

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The present invention is directed to an innate DNA sequence within the complete genome sequence ofsp. SE50/110 which resembles the structure of an actinomycete integrative and conjugative element (AICE). Related AICEs were used for establishing genetic manipulation tools for other bacteria in the past. In this document, we describe the unique features of the specific AICE found insp. SE50/110 which are clearly distinct from any other known AICE as a whole, but share minor parts with varying sequence similarity with other characterized AiCEs from other species.

The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the alr gene, and a host-vector system having a coryneform host bacterium in which the alr gene is present in attenuated form and a vector which carries at least the alr gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

Coryneform host-vector systems comprising a chromosomal ALR gene which is attenuated or eliminated and methods of using

Nucleotide sequences encoding alanine racemase from coryneform

The invention relates to an isolated polynucleotide from coryneform bacteria containing at least one polynucleotide sequence selected from the group consisting of

Nucleotide sequence encoding the dapC gene and process for the production of L-lysine

Disclosed herein is an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO:2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO:2 c) polynucleotide which is complementary to the polynucleotides of a) and b) and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acid with amplification of the zwa1 gene in the coryneform bacteria employed.

Process for the preparation of l-amino acids

The present invention relates to an isolated polynucleotide coding for the lrp gene and to a process for the fermentative production of L-amino acids by altering the expression of this gene.

Nucleotide sequence encoding corynebacterium glutamicum leucine response regulatory protein

The present invention is directed to nucleotide sequences coding for a bacterial enolase enzyme. These sequences may be used in improved methods for the fermentative preparation of amino acids using coryneform bacteria.

Nucleotide sequences which code for the eno gene

The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of

Polynucleotide sequences which encode the zwa1 gene

The present invention refers to abacterial strain which new isolated and identified secD and/or secF gene(s) is/are genetically modified in a way that secretion of the bacterial strain is enhanced, the protein- and polynucleotide sequences of these genes, a plasmid containing at least one of these genes and the use of such a bacterial strain for production of a desired substance or in a reporter system.

Corynebacterium glutamicum strain with enhanced secretion activity

The invention relates to a process for the preparation and improvement of D-pantothenic acid-producing microorganisms by amplification of nucleotide sequences which code for ketopantoate reductase, in particular the panE gene, individually or in combination with one another, and optionally additionally of the ilvC gene, the microorganisms containing these nucleotide sequences, and a process for the preparation of D-pantothenic acid comprising fermentation of these microorganisms, concentration of pantothenic acid in the medium or in the cells of the microorganisms, and isolation of the D-pantothenic acid.

Process for the preparation of pantothenic acid by amplification of nucleotide sequences which code for the ketopantoate reductase

Plasmids from Corynebacterium glutamicum and use thereof

The present application is directed to a diaminopimelate epimerase from and to polynucleotides encoding this enzyme. The gene has been given the designation &ldquo;dapF.&rdquo;

Polynucleotide encoding a diaminopimelate epimerase from Corynebacterium glutamicum

Growing community of inventors

Bielefeld, Germany

Jörn Kalinowski