Helixbind, Inc.

Qiagen Mansfield, Inc.

Biotype Gmbh

Jork Nolling

Alon Singer

Ranjit Prakash

David Steinmiller

Vincent Miles

Srinivas Rapireddy

Sergey V Voronov

Nadish Goyal

The present disclosure generally relates to the field of microbial pathogen detection and identification utilizing genomic sequence recognition.

Methods and devices for identifying microbial infections

The present disclosure generally relates to the field of ultrasensitive microbial pathogen detection and identification utilizing genomic sequence recognition.

Methods and devices for ultrasensitive direct detection of microorganisms

Methods and devices for isolating microbial cells from a sample, extracting eukaryotic DNA from a sample, and identifying the microbial species in the sample are disclosed herein.

Methods and devices for detecting and identifying microorganisms

Described are methods of reducing the incidence and/or magnitude of artifacts in denaturing nucleic acid capillary electrophoresis (CE). Methods and systems described serve to dismiss non-denatured DNA from the tip of the capillary after sample injection and prior to electrophoretic separation of loaded nucleic acids. Among the methods disclosed are the application of a brief reverse-polarity pulse to the capillary prior to separation but after removal of the capillary from the sample reservoir, and transiently heating the capillary to cause expansion of the separation matrix after removal of the capillary from the sample reservoir.

Reduced artifact denaturing capillary electrophoresis of nucleic acids

Described herein are approaches for the detection, identification, and/or quantification of target nucleic acids, including, but not limited to partially, substantially randomly, degraded target nucleic acids, in a biological sample, such as a formalin-fixed, paraffin-embedded (FFPE) sample. These approaches provide a means of detecting, identifying, and/or quantifying target nucleic acid molecules, including DNA and RNA molecules, further including RNAs of different classes, from the same sample, and in the same reaction, by using 'expander oligonucleotides,' as the term is defined herein, to convert fragments of target nucleic acids into discretely sized DNA fragments, each with a chosen length characteristic for the target nucleic acid from which it is derived.

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Hopedale, MA, United States of America

Jork Nolling