Covestro Deutschland Ag

Bayer Aktiengesellschaft

Jorgen Magnus

Amgad Salah Moussa

Gernot Jaeger

Helmut Brod

Guenter Olf

Joerg Kauling

Swantje Behnken

Giulio Lolli

Andre Pastor

Juri Seletzky

Georg Sprenger

Jung-Won Youn

Mohammad Yalfani

Gernot JÄGER

Günter Olf

The invention provides a recombinant microbial host cell capable of converting a raw material comprising a fermentable carbon substrate to o-aminobenzoate biologically. The invention further provides a method for producing aniline, comprising the steps of: a) producing o-aminobenzoate by fermentation of a raw material comprising at least one fermentable carbon substrate using the recombinant microbial host cell of the capable of converting said raw material comprising at least one fermentable carbon substrate to o-aminobenzoate biologically, wherein said o-aminobenzoate comprises anthranilate anion, b) converting said o-aminobenzoate from said anthranilate anion to anthranilic acid by acid protonation, c) recovering said anthranilic acid by precipitation or by dissolving in an organic solvent, and d) converting said anthranilic acid to aniline by thermal decarboxylation in an organic solvent.

Recombinant strain producing O-aminobenzoate and fermentative production of aniline from renewable resources via 2-aminobenzoic acid

The invention relates to a method for producing aniline, comprising the steps of: a) providing o-aminobenzoate, wherein said o-aminobenzoate comprises anthranilate anion and a suitable cation, b) converting said anthranilate anion to aniline by thermal decarboxylation in the presence or absence of a catalyst, c) extracting the aniline produced in step b) in an organic solvent at least once, and d) purifying the aniline produced in steps b) and c) by distillation, wherein said distillation produces aniline and a water phase.

Production of aniline via anthranilate

The invention relates to a separator for retaining and recirculating cells in a continuous-flow or batch-flow type plastic bag or bottle, which can preferably be operated outside of a bioreactor. Additionally, the invention relates to a method for retaining and recirculating cells within or outside a bioreactor. The invention further relates to a method for producing the separator according to the invention.

One-way separator for retaining and recirculating cells

The invention relates to a method of generating a recombinant host strain for producing phenol, comprising the steps of a) providing a host comprising chorismate, b) transforming the host with a first nucleic acid sequence comprising ubiC (SEQ ID NO: 1) encoding chorismate lyase that converts chorismate to 4-hydroxybenzoate, and c) transforming the host with a second nucleic acid sequence encoding an oxygen-tolerant 4-hydroxybenzoate decarboxylase that converts 4-hydroxybenzoate to phenol, thereby generating a recombinant host that is capable of producing phenol under aerobic conditions, wherein step b) and step c) are carried out simultaneously or sequentially. The invention also provides the recombinant host strain for producing phenol obtainable by the aforementioned method, as well as a method of producing phenol in the recombinant host strain.

Method for producing phenol from renewable resources by fermentation

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Dusseldorf, Germany

Jorgen Magnus