Biogen Idec Ma Inc.

Biogen Idec, Inc.

Biogen Ma Inc.

Jorg Thommes

Alan M Sonnenfeld

John P Pieracci

Lynn Conley

Marc Bisschops

Joydeep Ganguly

Asif Ladiwala

Maarten Pennings

Paul Cunnien

Basav Ghosh

Robert Song

Provided are methods of separating an immunoreactive compound from at least one immaterial component, using a simulated moving bed ('SMB') system and a SMB apparatus for use in these methods. Also provided are purified immunoreactive compounds prepared using the SMB methods and apparatus and methods of treatment with the purified immunoreactive compounds.

Method of purifying polypeptides by simulated moving bed chromatography

The present invention provides automated systems and methods for monitoring column performance in process chromatography, and applications thereof. In an embodiment, column performance is monitored by generating a plurality of process values such as, for example, conductivity values or pH values with a detector during a chromatography step transition between a first mobile phase liquid and a second mobile phase liquid. The process values are transformed to form transformed process values in which noise present in the process values is suppressed. Column performance parameters are calculated based on the transformed process values and displayed during movement of the second mobile phase liquid through the chromatography column. The displayed performance parameters enable an operator to make a determination, for example, regarding the quality of the chromatography column packing and whether to continue the chromatography process or stop the chromatography process until the chromatography column can be repacked or replaced.

Automated system and method for monitoring chromatography column performance, and applications thereof

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San Diego, CA, United States of America

Jorg Thommes