The University of Arizona

The United States Government As Represented by the Department of Veterans Affairs

Avery Therapeutics, Inc.

Jordan J Lancaster

Steven Goldman

Steve Goldman

Ikeotunye Royal Chinyere

Russell Witte

Jen Watson Koevary

Kyle Weigand

Jennifer W Koevary

Jennifer Watson Koevary

Disclosed herein are contractile cell constructs, methods for using them to treat disease, and methods for making them.

Muscle cell patches and uses therefor

Engineered tissue compositions for supporting cell growth, maintenance, and/or differentiation featuring a scaffold, extracellular matrix (ECM) material, and optionally a population of ECM-generating cells such as fibroblasts. The tissue compositions may be used for supporting seeded cells of a particular cell type of interest such as cells related to skeletal muscle, smooth muscle, cardiac tissue, gastrointestinal tissue, etc. The tissue compositions with seeded cells may develop into functional tissues, which may have the potential to provide a tissue graft for therapeutic purposes or a valuable model for in vitro assays.

Three dimensional tissue compositions and methods of use

The present invention provides compositions and methods for improving cardiac function. Specifically, the present invention provides compositions and methods for treating a subject having a disorder or condition associated with aberrant cardiac tissue function, comprising contacting a patient having a disorder or condition associated with aberrant cardiac tissue function with a construct, or a construct associated with therapeutic cells, or a construct associated with fibroblast cells and therapeutic cells.

Compositions and methods for improving cardiac function

The present invention provides methods for cellular seeding onto three-dimensional fibroblast constructs, three-dimensional fibroblast constructs seeded with muscle cells, and uses therefore.

Cellular seeding and co-culture of a three dimensional fibroblast construct

Systems are provided for assessing the likelihood that a sample of cardiac tissue will spontaneously exhibit disordered electrical activity. These systems induce ventricular tachycardia or other disordered electrical activity in a sample of human and/or animal cardiac tissue either in vivo or in vitro. This system can be used to assess the ability of various pharmaceuticals, genetic modifications, electrical pacing, surgical ablation, or other therapeutic interventions to prevent or halt such disordered electrical activity. This system detects electrical activity from a plurality of points on the surface of the sample of cardiac tissue and generates one or more maps of monophasic action potential amplitude, monophasic action potential duration, local field amplitude, or other electrophysiological parameters of the cardiac tissue. These maps are then used to assess the likelihood that the sample will spontaneously exhibit disordered electrical activity and/or to assess the effect of a therapeutic intervention on that likelihood.

Cardiac electrophysiologic mapping and stimulation

Disclosed herein are contractile cell constructs comprising contractile cells, or progenitors thereof, adhered to a surface of a three dimensional fibroblast containing scaffold (3DFCS) and methods for using them to treat disease. In one aspect, the present invention provides methods for preparing a contractile construct, comprising (a) seeding immature contractile cells onto the surface of a three dimensional fibroblast containing scaffold (3DFCS) to produce a contractile construct; and (b) culturing the contractile construct under conditions to promote differentiation of the immature contractile cells into mature contractile cells, wherein the mature contractile cells form striations. In a further aspect, the invention provides methods for treating a disorder characterized by a lack of functioning contractile cells, comprising contacting a patient with a contractile cell-based disorder with an amount effective to treat the disorder with the construct of any embodiment or combination of embodiments of the invention.

Growing community of inventors

Tucson, AZ, United States of America

Jordan J Lancaster