Nch Corporation

Nch Life Sciences LLC

Jordan E Church

Charles J Greenwald

Gabriel F K Everett

Amanda K Rosmarin

Daniel Aberle

Judy Pruitt

George Aboagye

Lester Levy

Haibo Cao

Christopher Zetena

Kelly A Gillespie

Michael J Schuster

Michael Paloian

Judith Gayle Pruitt

Skylar Rae White

Christopher J Laney

Nicole Faris

Joshua Medford

Gabby Everett

Jr Levy

Gabriel Fk Everett

Gabriel F Everett

A system and method for incubating a nutrient spore composition to at least begin the germination process at a point-of-use for small-scale farm applications. The composition comprises solid or powdered L-amino acids, a buffer, optionally D-glucose and/or D-fructose, optionally potassium, andspores in a pre-measured filter packet. The method comprises adding a small amount of hot water in a temperature range of 60° C. to 80° C. to the filter packet for an incubation period of around 2 to 60 minutes to form an incubated bacteria solution that is discharged to a farm application. A drip-style coffee maker or similar system may be used to add hot water to the filter packet. The incubated bacteria solution is dispensed to animal drinking water, plants/crops, or an aquaculture pond.

System and method for using a single-serve nutrient spore composition for small scale farm applications

A composition and method for activating probiotic spores in food and beverage products, such as steeped teas, coffee, soups, and sauces. A nutrient-germinant composition comprises one or more L-amino acids, optionally one or more buffers to maintain the pH of the composition when added to water in a range of around 6-8, optionally D-glucose, D-fructose, or both D-glucose and D-fructose, and optionally, an osmoprotectant. The nutrient-germinant composition, one or more species ofspores, and a food or beverage product may be pre-mixed in any combination. Water is added to the mixture and heated to a temperature range of 42 to 100&#xb0; C. to germinate the probiotic spores prior to being consumed.

Composition and method for germinative compounds in probiotic food and beverage products for human consumption

Metastable state spore incubation mixing systems are described. An example system includes a spore container to store spores, a nutrient container, an arrangement of valves and tubes, a reciprocating pump, a mixing tube, and a holding tank. In a drawing phase of the system, a controller can control the reciprocating pump to draw a ratioed volume of the spores, the nutrients, and water through the valves and tubes. During an expelling phase of the system, the controller can control flow control valves to direct the spores, nutrients, and water through the mixing tube and into the holding tank. The controller can also direct a heater to heat the mixture in the holding tank to a predetermined temperature. Once the mixture reaches the temperature, the controller can also direct the system through a number of other phases of operation, including cooling and purging phases.

Metastable state mixing

A method for improving the quality of pond water used in aquaculture applications by adding to the pond water active bacteria that are preferably germinated from spores on site using a nutrient-germinant composition and an incubation method for increased spore germination efficiency, in combination with a nitrification enhancement agent such as calcium carbonate or calcified seaweed, and an optional reaction surface area modifier such as calcified seaweed or plastic or metal particles or fragments. The nutrient-germinant composition comprises L-amino acids, D-glucose and/or D-fructose, a phosphate buffer, an industrial preservative, and may include bacteria spores (preferably of one or morespecies) or they may be separately combined for germination. The incubation method comprises heating a nutrient germinant composition and bacteria spores, to a temperature range of 35&#xb0; C. to 60&#xb0; C. for around 2 to 60 minutes to produce an incubated bacteria solution that is discharged to the aquaculture application.

Method for improving quality of aquaculture pond water using a nutrient germinant composition and spore incubation method

A probiotic composition and method for activating probiotic spores for consumption by a human to reduce inflammation in or treat inflammatory conditions in the gut. A probiotic composition comprises a nutrient-germinant composition, one or more species ofspores, and optionally a food or beverage product, which are mixed or pre-mixed in any combination. A nutrient-germinant composition comprises one or more L-amino acids, optionally a source of potassium ions, and optionally one or more buffers, if the source of potassium ions is not also a buffer. A method of activating the spores comprises heating the probiotic composition or food or beverage containing the probiotic composition to a temperature range of around 42&#xb0; C.-100&#xb0; C., more preferably 70&#xb0; C.-85&#xb0; C. prior to being administered to ingested. Dosing the probiotic composition at around 1 to 4 grams per day over a treatment cycle can reduce indicators of inflammation by at least 10-20% or more.

Method of reducing gut inflammation reduction in humans by consuming a heated probiotic composition

A nutrient-germinant composition to aid in spore germination and a method for increased spore germination efficiency. The composition comprises L-amino acids, D-glucose and/or D-fructose, a phosphate buffer, an industrial preservative, and may include bacteria spores or they may be separately combined for germination. The method comprises providing a nutrient-germinant composition and bacteria spores, preferably of one or morespecies, and heating to a preferred elevated temperature range of 41&#xb0; C. to 44&#xb0; C. for an incubation period of around 2 to 60 minutes. The nutrient-germinant composition is preferably in a concentrated liquid form that is diluted just prior to initiating the germination/incubation method at the point of use. The method may also include dispensing a germinated spore solution to a point-of-use/consumption, such as animal feed, water, or bedding, or a wastewater system or drain.

Nutrient rich germinant composition and spore incubation method

A method for treating and improving the quality of pond water used in growing aquatic species for aquacultural applications by adding to the pond water active bacteria that are preferably grown on site in combination with a nitrification enhancement agent such as calcium carbonate or calcified seaweed, and an optional reaction surface area modifier such as calcified seaweed or plastic or metal particles or fragments.

Method for improving quality of aquaculture pond water

Growing community of inventors

Dallas, TX, United States of America

Jordan E Church