Koninklijke Philips Corporation N.v.

Minicare B.v.

Joost Lambert Max Vissers

Toon Hendrik Evers

Wendy Uyen Dittmer

Femke Karina De Theije

Peggy De Kievit

Ricky Kamps

David Walterus Cornelis Dekkers

The present invention relates to a method for detection of at least one target molecule in a blood sample comprising at least the following steps: (a) providing a blood sample; (b1) contacting said sample with an antioxidant and applying said sample to a filter to separate the blood cells from said sample; and/or (b2) applying said sample to a filter to separate the blood cells from said sample, wherein said filter comprises an antioxidant; (c) contacting said sample with a first binding molecule in the cartridge, wherein said first binding molecule is attached to a magnetic particle, and wherein said first binding molecule is capable of specifically binding to said at least one target molecule; (d) contacting said sample with a second binding molecule, wherein said second binding molecule is attached to a sensor surface, and wherein said second binding molecule is capable of specifically binding to said at least one target molecule; and (e) detecting the magnetic particles on the sensor surface. The present invention also relates to the use of an antioxidant in the process of detection of at least one target molecule in a blood sample as well as to cartridges for insertion into an assay device for detection of at least one target molecule in a blood sample.

Use of antioxidants in methods and means for detection of target molecules in a blood sample

The present invention relates to a method for measuring Troponin I in a sample comprising the steps of providing a sample, contacting the sample with a monoclonal anti-Troponin I antibody coupled to a magnetic label, contacting the sample with a polyclonal anti-Troponin I antibody coupled to a sensor surface and detecting the magnetic label on the sensor surface. The invention further relates to a device and a cartridge for measuring Troponin I in a sample.

Assay for Troponin I using magnetic labels

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Nijmegen, Netherlands

Joost Lambert Max Vissers