Leica Mikrosysteme Gmbh

Biogenium Microsystems Oy

Joose Kreutzer

Paul Wurzinger

Rainer Wogritsch

Panu Jaakkola

Pasi Kallio

Krista Rantanen

Olli Tanhuanpää

A method including one or more steps of a procedure for preparing a microscopic sample for a high-pressure freezing process is provided, wherein the sample is provided using an arrangement comprising a middle plate of a high-pressure freezing cartridge and an incubation chamber, wherein the middle plate is attached to a lower surface of a bottom of the incubation chamber by an adhesive and detachable from the incubation chamber by effecting a relative movement between the middle plate and the incubation chamber, wherein the sample is provided on an enclosing element which is fitted into an opening of the middle plate, and wherein, when the sample is to be subjected to the high-pressure freezing process, the relative movement between the middle plate and the incubation chamber is effected, thereby detaching the middle plate with the sample from the incubation chamber. Means to implement the method are also part of the present disclosure.

Preparation of a sample for high pressure freezing

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Tampere, Finland

Joose Kreutzer