Life Technologies Corporation

Applied Biosystems, LLC

Cellares Corporation

Joon Mo Yang

David M Liu

Mark F Oldham

Charles Stuart Vann

Joy Roy

Dennis Lehto

Ian A Harding

Yuh-Min Chiang

Nigel P Beard

Umberto Ulmanella

Alexander A Dromaretsky

Sergey V Ermakov

Maryam Shariati

John R Van Camp

Min Yue

Jon A Hoshizaki

Patrick D Kinney

Steven W Sherwood

Huan L Phan

Aldrich Lau

Bharat S Thakkar

Michele Elizabeth Wisniewski

Hans Fuernkranz

Joshua Shen

Yiming Xu

Stephen G Carmichael

John Van Camp

The present disclosure relates to systems and methods for monitoring pressure during automated cell processing. An illustrative method for monitoring pressure within a fluid device of an automated cell processing system includes coupling a fluid device having an air vent to a cell processing instrument. Next, the method includes transferring liquid between the fluid device and the instrument, determining a liquid transfer rate between the fluid device and instrument and an airflow rate through the air vent of the fluid device, and estimating an internal pressure of the fluid device based on the airflow rate and the liquid transfer rate. The fluid device may be a liquid transfer device, the instrument may be a liquid transfer instrument, and the system may facilitate liquid transfer between the liquid transfer device and a cell processing cartridge via the liquid transfer instrument.

Monitoring air pressure within a cell processing system

Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials.

Systems and methods for multiple analyte detection

A method for sorting cells in a biological sample comprising a first type of cells and a second type of cells may comprise introducing the biological sample into a chamber comprising a first surface and a second surface, wherein the first surface is associated with a transparent electrode and the second surface is associated with a photoconductive portion of an electrode. The method may further comprise moving incident light and the photoconductive portion relative to one another so as to illuminate regions of the photoconductive portion and modulate an electric field in the chamber in proximity to the illuminated regions. The method may further comprise separating the first type of cells from the second type of cells in the chamber via dielectrophoretic movement of the first type of cells and the second type of cells caused by the modulated electric field, wherein a dielectrophoretic characteristic of at least one of the first type of cells and the second type of cells has been modified.

Devices and methods for optoelectronic manipulation of small particles

Assemblies for and methods of coupling a microtiter plate and receptacle for centrifugation of liquid from the microtiter plate to the receptacle are provided. In some embodiments, a coupling frame can be used. In other embodiments, the microtiter plate couples directly to the receptacle. In some embodiments, relative motion between the receptacle and the microtiter plate is limited in the x-y plane. In some embodiments, relative motion between the receptacle and the microtiter plate is limited in the x-z plane. In some embodiments, relative motion between the receptacle and the microtiter plate is limited in the y-z plane.

Methods and assemblies for collecting liquid by centrifugation

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Redwood City, CA, United States of America

Joon Mo Yang