Korea Research Institute of Bioscience and Biotechnology

Joo Hyung Heo

Sang Ki Rhee

Hyun Ah Kang

Hye Yun Moon

Doo Byoung Oh

Jeong Seok Park

Eun Jung Kim

Moo Woong Kim

Sang-Ki Rhee

Hyun-Ah Kang

Moo-Woong Kim

Hyun Ah Department Of Life Science Kang

Hye Yun Department Of Life Science Moon

The present invention relates to a process for producing a human-type glycoprotein having reduced glycosylation by genetically manipulating an enzyme involved in glycosylation using asystem. In detail, the present invention relates to a process for producing a human-type glycoprotein by identifying a dolichyl-phosphate-mannose dependent &#x3b1;-1,3-mannosyltransferase gene from, constructing amutant strain producing a glycoprotein exhibiting reduced glycosylation by disrupting the identified gene, and subjecting the mutant strain to various genetic manipulations for the synthesis of human-type glycan.

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The present invention relates to a process for producing a human-type glycoprotein having reduced glycosylation by genetically manipulating an enzyme involved in glycosylation using asystem. In detail, the present invention relates to a process for producing a human-type glycoprotein by identifying a dolichyl-phosphate-mannose dependent &#xa5;&#xe1;-1,3-mannosyltransferase gene from, constructing amutant strain producing a glycoprotein exhibiting reduced glycosylation by disrupting the identified gene, and subjecting the mutant strain to various genetic manipulations for the synthesis of human-type glycan.

Disclosed are a novelgene coding for &#x3b1;-1,6-mannosyltransferase initiating outer chain elongation, anmutant strain having a deficiency in the gene, and a process for producing a recombinant glycoprotein using such a mutant strain.

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Daejeon, South Korea

Joo Hyung Heo