Thermo Fisher Scientific Oy

Joni Rannisto

Lauri Peltosaari

Leena Valmu

Kelly Flook

Johan Finell

Ville Saarainen

Roger Grist

Anssi Rantakari

Mikko Koskinen

Suvi Ravela

The present invention includes a novel method and system for identification of microorganisms in samples that proteins and other biological material from non-microorganism sources (e.g., proteins of mammalian origin) that can interfere with identification of the microorganisms. The methods and systems described herein include use of a single-use chromatography medium to purify intact proteins prior to mass spectrometry analysis. The chromatography medium and the methods described herein can rapidly and efficiently remove of a substantial portion of interfering biological material (e.g., mammalian proteins) from a crude cell lysate while preserving high signal strength and removing enough of the interfering protein(s) to allow for identification of the microorganism(s) by mass spectrometry analysis.

Mass spectrometric methods and kits to identify a microorganism

A sample vessel () for transferring and analyzing a sample with analyzer instrument, which sample vessel comprises an elongated vessel body () for containing the sample and a hinged cap () connected in top end area of the vessel body, and the cross-section of the vessel body () on a transversal plane in relation to the lengthwise center axis of the vessel body the inner surface of the vessel body is rotationally asymmetrical in relation to the lengthwise center axis, wherein the sample vessel () comprises a surface () extending upwards from the vessel body, which surface partially surrounds the edge of the cap (), when the cap is in closed position.

Sample vessel

Described are extraction columns and systems that include extraction columns. The extraction column includes a column body that has a reservoir portion, an extraction media portion, and a collar portion. The reservoir portion includes an inlet and a reservoir. The extraction media portion includes an elongated sleeve having an inner surface defining a cavity. The extraction media portion has an inlet end in fluid communication with the reservoir and an outlet end. An extraction medium is disposed in the cavity of the elongated sleeve. The collar portion extends axially in a common direction with the elongated sleeve. The collar portion has a terminal end which is spaced apart from the outlet end of the elongated sleeve and which extends axially at least to a plane defined by the outlet end of the elongated sleeve.

Extraction column

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Kerava, Finland

Joni Rannisto