Massachusetts Institute of Technology

National University of Singapore

The General Hospital Corporation

Nanyang Technological University

The Broad Institute, Inc.

Massachusetts General Hospital Corporation

National University Hospital (s) Pte Ltd.

Universidade Federal De Paraiba

Jongyoon Han

Sung Jae Kim

Han Wei Hou

HiongYap Gan

Lidan Wu

Sung Hee Ko

Chwee Teck Lim

Rhokyun Kwak

Guofeng Guan

Ali Asgar Bhagat

Weng Kung Peng

Bumjoo Kim

Hyunryul Ryu

Hyungkook Jeon

Siwon Choi

Kyungyong Choi

Bader Shafaqa Al-Anzi

Joel Voldman

Hao-Wei Su

Krystyn J Van Vliet

Roby Bhattacharyya

Galit Alter

Majid Ebrahimi Warkiani

Ying-Chih Wang

Chong Jin Ong

Wong Cheng Lee

Jianping Fu

Frederico Barbosa De Sousa

Hansen Bow

Patrick Abgrall

Dustin Moon

Lan Chen

Junghyo Yoon

Hyuckjin Kwon

Kah Ping Andy Tay

Tze Ping Loh

Aniruddh Sarkar

Javier Lopez Prieto

Deb Hung

Bee Luan Khoo

Minseok Kim

Chao Yu Peter Chen

The invention is directed to a scalable concentration device and method of use thereof based on electrokinetics.

Electrokinetic-based concentrator device and method

Described is a multi-dimensional double spiral (MDDS) microfluidic device comprising a first spiral microchannel and a second microchannel, wherein the wherein the first spiral microchannel and second spiral microchannel have different cross-sectional areas. Also described is a device comprising a multi-dimensional double spiral and system for recirculation. The invention also encompasses methods of separating particles from a sample fluid comprising a mixture of particles comprising the use of the multi-dimensional double spiral microfluidic device.

Multi-dimensional double spiral device and methods of use thereof

The present invention provides return flow ICP and ED systems and methods that can be used for water desalination and/or concentration of a wide range of target brine and other aqueous and contaminated streams.

Return flow system for ion concentration polarization (ICP) desalination

This disclosure provides an apparatus and a method for quickly, efficiently and continuously fractionating biomolecules, such as DNAs and proteins based on size and other factors, while allowing imaging of the separated biomolecules as they are processed within the apparatus. The apparatus employs angled nanochannels to first preconcentrate and then separate like molecules. Its embodiments offer improved detection sensitivity and separation resolution over existing technologies and multiplexing capabilities.

One-step protein analysis using slanted nanofilter array

A system for intra-operative blood salvage autotransfusion is provided. The system comprises at least one inlet configured to receive whole blood of a patient; at least one curvilinear microchannel in fluid flow connection with the at least one inlet, the at least one curvilinear microchannel being adapted to isolate circulating tumor cells in the whole blood, based on cell size, along at least one portion of a cross-section of the at least one curvilinear microchannel; and at least two outlets in fluid flow connection with the at least one curvilinear microchannel, at least one outlet of the at least two outlets being configured to flow the circulating tumor cells isolated from the whole blood, and at least one other outlet of the at least two outlets being configured to flow at least a portion of a remainder of the whole blood, cleansed of the isolated circulating tumor cells, for return to the patient.

System and method for inertial focusing microfiltration for intra-operative blood salvage autotransfusion

This invention describes a one-step technique for the simultaneous label-free detection and concentration of blast cells from a blood sample. Enrichment of blast cells is achieved using a closed loop microfluidics system, allowing continuous removal of waste and non-target cells to generate concentrated samples of high purity without the need for specific targeting of proteins by antibodies. The technique is highly effective for samples which cannot be purified in a single run. The application of detecting rare blast cells for monitoring minimal residual disease in leukemia patients is demonstrated. The sensitivity of the invention promotes the detection of blast cells in blood samples of early-stage patients.

Liquid biopsy detection of leukemia using closed-loop microfluidics

The present invention encompasses a micro-fluidic system having a closed-loop configuration in which inertial micro-fluidic separation of particles and/or cells is continuously repeated by feeding part of the output back to the input so that the purity and/or concentration of the particles and/or cell is maximized. The invention also includes methods of using the micro-fluidic system.

Particle isolation/enrichment using continuous closed-loop micro-fluidics

Scalable, high throughput and power-efficient electromechanical lysis using low electric potential, which can be used for harvesting valuable intracellular biomolecules (DNA, RNA, and proteins) and metabolites (e.g., biodiesels, bioplastics, antibiotics, and antibodies), and for sterilizing large volume solutions (e.g. disinfection of bacterial contaminated drinking water). The method can be directly integrated with other microfluidic devices for all-in-one, fully integrated total-analysis systems for various bacterial (and cellular) studies and clinical applications.

Electromechanical lysis of bacterial pathogens using ion concentration polarization

In accordance with one aspect of this disclosure, there is provided a device for performing magnetic resonance relaxometry. The device comprises a radio-frequency spectrometer comprising at least one field-programmable gate array chip; a power amplifier electrically connected with the radio-frequency spectrometer and amplifying an electrical output of the radio-frequency spectrometer, thereby producing an amplified electrical signal comprising between about 0.1 Watts and about 10 Watts power; a duplexer configured to isolate the radio-frequency spectrometer from the amplified electrical signal during a receiving mode of the device; a radio-frequency detection probe configured to transmit radio-frequency electromagnetic radiation to excite nuclei under resonance during a transmission mode of the device, the radio-frequency detection probe comprising a detection coil comprising an inner diameter of less than about 1 millimeter; and at least one magnet supplying an external magnetic field to a detection region of the radio-frequency detection probe, the external magnetic field being less than about 3 Tesla.

Biosensor, palm-sized device and method based on magnetic resonance relaxometry

A low-cost and bench-top magnetic resonance relaxometer can be used for ex-vivo biochemical stress tests on plasma/erythrocytes, enabling deep-phenotyping of an individual's oxidative status, susceptibility and capacity.

Micro magnetic resonance relaxometry

A water stream is passed between two juxtaposed similar ion exchange membranes (AEMs or CEMs), forming an ion depletion and ion enrichment zones when an electric field is applied. As cations are selectively transferred through the CEMs, for example, anions are relocated in order to achieve electro-neutrality, resulting in the concentration drop (increase) in ion depletion (enrichment) zone. Trifurcation of the output channel allows collection of concentrated, dilute and intermediate streams, with the intermediate stream serving as input to the next stage of a serialized implementation.

Purification of ultra-high saline and contaminated water by multi-stage ion concentration polarization (ICP) desalination

In accordance with an embodiment of the invention, there is provided a method for: a) high-throughput, multiplexed, affinity-based separation of proteins&#x2014;especially low abundance proteins&#x2014;from complex biological mixtures such as serum; and b) high-throughput, multiplexed, affinity-based separation of cells&#x2014;especially rare cells&#x2014;from complex biological mixtures such as blood or blood fractions. The separation of proteins or cells is achieved based on differential binding to affinity-capture beads of different sizes and then sorting the protein-bound or cell-bound beads using the concept of centrifugal-induced Dean migration in a spiral microfluidic device. This method enables continuous-flow, high throughput affinity-separation of milligram-scale protein samples or millions of cells in minutes after binding. This is particularly applicable to the isolation of antigen-specific antibodies from polyclonal sera and antigen-specific immune cells or circulating tumor cells from blood, both of which are otherwise highly labor-intensive and expensive to perform.

System and method for multiplexed affinity purification of proteins and cells

A system includes a microfluidic device configured to isolate one or more particles from a mixture, a flow rate matching device configured to match flow rate of the microfluidic device with flow rate of an electrical measurement device configured to measure an electrical property of the isolated particles, and an electrical measurement device configured to measure an electrical property of the isolated particles.

Integrated electrical profiling system for measuring leukocytes activation from whole blood

Between two juxtaposed similar ion exchange membranes (AEMs or CEMs), an ion depletion zone and ion enrichment zone are generated under an electric field. As cations are selectively transferred through the CEMs, for example, anions are relocated in order to achieve electro-neutrality, resulting in the concentration drop (increase) in ion depletion (enrichment) zone. The use of a sacrificial metal anode allows electrocoagulation (EC) concurrently with ICP thereby permitting concentration of both ionic and non-ionic impurities to occur at the same time within the same cell or device.

Ion concentration polarization-electrocoagulation hybrid water treatment system

Growing community of inventors

Bedford, MA, United States of America

Jongyoon Han