Korea Basic Science Institute

Jong Shin Yoo

Hyun Sik Kim

Myoung Choul Choi

Jin Young Kim

Yeong Hee Ahn

Seung Yong Kim

Ju Yeon Lee

Gun Wook Park

Sung Hwan Kim

Hyun Kyoung Lee

Kun Cho

Eun Sun Ji

Soohyun Kim

Yeon Suk Choi

Jeong Min Lee

Jae-Han Kim

Jae Yong Lee

Dong Lak Kim

Hyun Joo An

Kyung Hoon Kwon

Kyu Hwan Park

Kyung-Hoon Kwon

Stefan Karl-Heinz Stahl

Duk Jin Kang

Park Min Shin

A Leum Park

Kwang Hoe Kim

Kun 0Ho

The preset invention relates to a high resolution mass spectrometry based novel bioinformatics platform for the identification of glycation proteins and advanced glycation end-product. Particularly, the bioinformatics platform of the present invention facilitates an efficient and accurate investigation of quantitative changes of glycation proteins and advanced glycation end-products which are included in various types of samples but had not been informed yet, and uses a high resolution mass spectrometry, and thereby can be effectively used for the prediction or diagnosis of a disease including cancer by examining a disease marker in a sample.

Mass spectrometry based bioinformatics platform for high-throughput identification of glycation proteins and advanced glycation end-products

The present invention relates to a bioinformation processing analysis method for the identification and quantification of O-linked glycopeptide using high resolution mass spectrum. Particularly, according to the bioinformation processing analysis method of the present invention, the quantitative changes of O-linked glycopeptide containing non-informed sugar chains included in various samples can be efficiently and accurately analyzed; the prediction or diagnosis of disease including cancer can be made easy by using a high resolution mass spectrometer; or the investigation of O-linked glycopeptide structure of a therapeutic glycoprotein can be efficiently achieved.

Bioinformatics platform for high-throughput identification and quantification of O-glycopeptide

The present invention relates to a diagnosis method of liver cancer using mass spectrometry of &#x3b1;-fetoprotein derived glycopeptide. Particularly, according to the AFP glycopeptide analysis method of the invention, fucosylation rate of the glycopeptide having the sequence composed of Val-Asn-Phe-Thr-Glu-Ile-Gln-Lys is analyzed to diagnose liver cancer in the early developmental grade. In particular, fucosylation rate is higher in the liver cancer patients than in other liver disease patients, so that the comparison of the fucosylation rate can be useful to diagnose or distinguish liver cancer from other liver diseases in the early HCC patients.

Diagnostic method of liver cancer using &#x3b1;-fetoprotein derived glycopeptides by mass spectrometry

The present invention relates to a more efficient and accurate method for the identification and quantification of comparatively low abundant glycopeptides, compared with general peptides, using mass spectrum obtained by using high resolution mass spectrometer. Therefore, the method of the present invention can be effectively used for the techniques for identification of biotherapeutics and diagnosis of cancer or disease by screening glycopeptide, the disease marker (Biomarker), from various samples.

Bioinformatics platform for high-throughput identification and quantification of N&#x2014;glycopeptide

A Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS) includes: an ionization source generating ions; a deceleration lens, on which the ions generated by the ionization source and spatially dispersed are incident, selectively decelerating the incident ions so as to decrease the distance between the ions; and an ion cyclotron resonance cell on which the ions passing through the deceleration lens are incident. By preventing dispersing of ions due to mass difference and converging the ions using the deceleration lens, the mass range that can be measured at one time can be extended. Also, measurement sensitivity can be improved since the ions are effectively introduced to the ICR cell.

Fourier transform ion cyclotron resonance mass spectrometer and method for concentrating ions for fourier transform ion cyclotron resonance mass spectrometry

The present invention relates to an apparatus and method for controlling a pipeline-type ion cyclotron resonance mass spectrometer, in which an ion trap unit of the ion cyclotron resonance mass spectrometer is capable of using two digitizers at the same time, thus enabling a measurement process for detecting an electrical signal which indicates the mass of ions corresponding to a specific purpose, and another measurement process for detecting another electrical signal which indicates the mass of ions corresponding to another specific purpose, to be simultaneously performed. Accordingly, it is an aim of the present invention to provide an apparatus and method for controlling a pipeline-type ion cyclotron resonance mass spectrometer, which can overcome the problems of time delay among control procedures, and can present a signal detection step wherein an excitation electrode is utilized to improve the sensitivity and speed of signal detection.

Apparatus and method for controlling a pipeline-type ion cyclotron resonance mass spectrometer

Provided are a controller and a control method for improving signal performance of an ion cyclotron resonance mass spectrometer. The controller and control method apply electric signals for causing ions injected into an ion trap of the ion cyclotron resonance mass spectrometer to be injected to the center of the trap as close as possible to trap electrodes, and adjust biased ion motion by appropriately adjusting signals of trap electrodes for causing the injected ions to make ion motion, thereby improving the fidelity of ion signals. The control method for improving signal performance of an ion cyclotron resonance mass spectrometer includes an ion position adjustment process and an ion signal detection process.

Controller and control method for improving signal performance of ion cyclotron resonance mass spectrometer

The present invention relates to a method for diagnosing cancer using information on aberrant glycosylation of glycoproteins, which is related with cancer progression. More particularly, the present invention relates to a peptide marker for cancer diagnosis and a method for diagnosing cancer using the peptide marker, wherein glycoproteins aberrantly glycosylated due to cancer incidence and progression is isolated using lectin; and marker peptides generated by hydrolysis of the glycoproteins isolated by the lectin is selected and quantified.

Method for diagnosing cancer using lectin

The present invention relates to a method for diagnosing cancer using information on the aberrant glycosylation of a glycoprotein. More precisely, the present invention relates to a cancer diagnosis peptide marker, which is screened by: a step of separating, using a lectin, a glycoprotein which is aberrantly glycosylated in accordance with the occurrence of cancer; and a step of selecting a hydrolyzed peptide marker derived from the aberrantly glycosylated glycoprotein by observing the quantitative changes in the separated glycoprotein. The present invention also relates to a method for diagnosing cancer using the peptide marker as an agent.

Cancer diagnosis marker using the aberrant glycosylation of a protein

A method for discovering pharmacologically active substances from natural products at high speed, including: obtaining an activity profile by testing pharmacological activity of a plurality of samples; obtaining mass profiles based on mass spectra resulting from analysis of the samples by mass spectrometry; and determining molecular weight of pharmacologically active substances by comparing and analyzing the activity profile and the mass profile. The method allows fast discovery of pharmacologically active substances by performing high resolution mass spectrometry for numerous components included in an extract sample of natural products and comparing with the activity test data. The information about the intensity of the activity of the pharmacologically active substances of the natural products allows effective utilization of the natural products.

Method for discovering pharmacologically active substance of natural products using high resolution mass spectrometry and pharmacological activity test

An apparatus for electrospray ionization may include: a platform including an inlet port, a first channel connected to the inlet port, a second channel connected to the first channel, and an outlet port connected to the second channel; a nebulizer provided in the first channel and configured to spray inert gas to a sample sprayed into the first channel through the inlet port; and a focusing lens provided in the second channel and configured to focus ions produced from the sprayed sample toward the outlet port.

Apparatus for electrospray ionization and method for electrospray ionization using the same

A Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS) is provided. A preamplifier is installed as nearest to an ion cyclotron resonance (ICR) trap as possible at a detector part in the mass spectrometer, and thermal noise generated at the preamplifier is minimized by means of a cryo-cooling system to increase a signal-to-noise ratio of ion detection signals such that an ultra-low amount of specimen can be detected, which was impossible in the related art.

Fourier transform ion cyclotron resonance mass spectrometer using a cryo-detection system

The present invention relates to a method for reconstructing protein database for identifying a protein and a method for screening a protein using the same, more precisely a method for reconstructing protein database, and a method for identifying a protein using the same. The method for reconstructing protein database and the method for identifying the protein of the invention are very useful for the investigation of endogenous proteins and their functions and interactions, and are further effectively used for the development of diagnostic and therapeutic agents for various diseases.

Method for reconstructing protein database and a method for screening proteins by using the same method

A tandem Fourier transform ion cyclotron resonance mass spectrometer is provided. In the mass spectrometer, the ions selected by a FT-ICR mass analyzer, which can perform an ion selection process and a mass measurement process with a time interval between the processes, are transmitted through an ion guide to a collision cell, which is located a predetermined distance from the FT-ICR mass analyzer, to split into fragment ions. The fragment ions are transmitted to the FT-ICR mass analyzer that measures the mass of the fragment ions. The fragment ions are generated in the collision cellestablished separately from the FT-ICR mass analyzeraccording to the mass spectrometer. Accordingly, It can solve various problems (e.g., the radius reduction of cyclotron motion of colliding ions, or the removal of periphery gas after generating the fragment ions) occurred in a tandem mass spectrometer using a conventional tandem-in-time mass analysis method. Also, a high resolution and with sensitivity measurement can be achieved. Moreover, when a reagent gas instead of a collision gas in the collision cell is injected, the gas phase reaction of the selected ions and the reagent gas can be observed, and the mass of the ions generated in the gas phase reaction can be measured.

Tandem fourier transform ion cyclotron resonance mass spectrometer

A differential pumping system in which a vacuum chamber is manufactured to have a plurality of vacuum pumps and conductance limit plates between the vacuum pumps. The differential pumping system in which an ion is implanted from an ionization source, passes through the vacuum chamber and is detected by a detection unit includes a plurality of vacuum pumps in the vacuum chamber and one or more conductance limit plates between the vacuum pumps, and the conductance limit plates form an angle less than 90&#xb0; with the transmission direction of the ion. According to such a constitution, higher degree of vacuum and the ion transmission efficiency are maintained without increasing the length of the vacuum chamber. Furthermore, it is possible to minimize the attenuation of an ion detection signal due to a collision with a neighboring neutral molecule, improving the sensitivity of an ion detection device.

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Daejeon, South Korea

Jong Shin Yoo