Life Technologies Corporation

Medimmune Limited

Jonathan Zmuda

Xavier De Mollerat Du Jeu

Christina M Strange

Wendy Irene White

Xin Yu

Chao Yan Liu

Chao Yan Lin

Jian Liu

Maya Yovcheva

Sara Barnes

Kenneth Thompson

Chang Choi

The present disclosure is related generally to systems and methods for high level expression of recombinant proteins from baculovirus in insect cells. In particular, the methods and systems described herein allow for high levels of baculovirus production in insect cells and/or high levels of protein production in insect cells using a chemically-defined, yeast lysate-free insect cell medium. The disclosure also relates to compositions and kits for culturing, transfecting, and/or producing recombinant protein in insect cells.

Chemically-defined baculovirus expression system

The instant technology relates to a production system to produce AAV vectors in a serum free suspension platform and at high titers. This technology uses reagents comprising media, cells, transfection reagent, AAV enhancer, and a lysis buffer, each of which is designed to provide maximal AAV production from suspension culture of mammalian cells, e.g. HEK293 cells. With this new system we are able to deliver up to about 2×10viral genomes per milliliter (vg/mL) of unconcentrated AAV vectors.

Suspension system for adeno associated virus production

The instant technology relates to a production system to produce AAV vectors in a serum free suspension platform and at high titers. This technology uses reagents comprising media, cells, transfection reagent, AAV enhancer, and a lysis buffer, each of which is designed to provide maximal AAV production from suspension culture of mammalian cells, e.g. HEK293 cells. With this new system we are able to deliver up to about 2&#xd7;10viral genomes per milliliter (vg/mL) of unconcentrated AAV vectors.

The present invention encompasses auto-antibodies associated with autoimmune disorders. The auto-antibodies may be used, for example, in methods of treating patients, methods of diagnosing patients, methods of monitoring disease progression of patients, and methods of prognosing patients.

Methods of treating lupus

Growing community of inventors

Frederick, MD, United States of America

Jonathan Zmuda