Genzyme Corporation

Watershed Informatics, Inc.

Jonathan Wang

Jason Walther

Neha Shah

Jiuyi Lu

Cheng Cheng

Timothy Allan Johnson

Mark Kalinich

Christopher Hwang

Seul-A Bae

Kevin Victor Chen

Alvin Wang

Yukun Ren

Jean McLarty

Timothy Johnson

Provided herein are methods of culturing a mammalian cell in a liquid medium including poloxamer-188 at a concentration of 1.8 g/L or at a greater concentration than 1.8 g/L more or a liquid medium that includes a poloxamer-188 concentration that is selected based on one or more factors selected from the group of: pore size, pore type, gas flow rate, viable cell density in the medium, and markers related to cell stress.

Methods of culturing a mammalian cell

Techniques for visual software development are disclosed, including: (a) generating, by a visual code editor, (1) a data model of a plurality of code elements in a set of source code and (2) a kernel for execution of the set of source code; (b) based on a subset of the set of source code, the data model, and the kernel, generating an adjustable graphical user interface (GUI) form control; (c) presenting the adjustable GUI form control in alignment with the set of source code in a GUI of the visual code editor; and (d) responsive to user input in the GUI to adjust the adjustable GUI form control: (1) modifying the subset of source code and the data model according to the user input; (2) updating the presentation of the set of source code in the GUI to reflect the modified subset of source code; and (3) executing the modified set of source code, thereby updating variable values within the kernel.

User interface controls for visual software modification

Techniques for visual software development are disclosed, including: generating, by a visual code editor, a data model of code elements in a set of source code; based on the data model, generating an adjustable graphical user interface (GUI) control corresponding to a subset of the set of source code; presenting the adjustable GUI control in a GUI of the visual code editor; and responsive to user input in the GUI to adjust the adjustable GUI control, modifying the set of source code according to the user input and executing the modified set of source code.

The disclosure provides a filtration system for a cell culture apparatus and a method of cell culture. The filtration system comprises a bioreactor vessel and two or more alternating tangential flow (ATF) filters connected in parallel. A failure in either filter is detected by an in-line sensor, and an automated response system functions to sequester the malfunctioning filter by stopping the flow of liquid media through the filter. Media flow through the remaining operable filters can be increased so that the rate of perfusion through the bioreactor remains relatively unchanged. Such a system may prevent issues that arise from ATF filter failures in conventional perfusion bioreactors, thereby improving the long-term viability of cell cultures.

Perfusion bioreactor with filtration systems

Provided herein are methods of predicting the effect of a concentration of a sensitizer on cell viability in a production bioreactor, methods of improving cell viability in a production bioreactor, methods of predicting cell viability in a production bioreactor, and methods for culturing a cell in a production bioreactor.

Methods for improving cell viability in a production bioreactor

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Bridgewater, NJ, United States of America

Jonathan Wang