Codexis, Inc.

Tate & Lyle Solutions USA LLC

Crosswalk Therapeutics, Inc.

Jonathan Vroom

Xiyun Zhang

Gjalt W Huisman

Nicholas John Agard

Benjamin Mijts

Nikki Dellas

Jovana Nazor

Jack S Liang

David Entwistle

Jeffrey C Moore

Oscar Alvizo

Da Duan

Mark A Huffman

Agustina Rodriguez-Granillo

Deeptak Verma

Stephanie Sue Galanie

Santhosh Sivaramakrishnan

Vesna Mitchell

Fernando Valle

Robert Osborne

Ravi David Garcia

Joyce Liu

Rama Voladri

Louis Clark

Birgit Kosjek

Shane Grosser

Matthew Truppo

Scott J Novick

Keith Allen Canada

David Elgart

James Nicholas Riggins

Mathew G Miller

Anna Fryszkowska

Xiang Yi

Nicholas M Marshall

Courtney Dianne Moffett

Grant S Murphy

Harvinder Chagger Maniar

Iman Farasat

Donald Scott Baskerville

Jay Russell

Nandhitha Subramanian

Kristian Karlshoej

Joshua N Kolev

Sandy M Gomes

Hao Yang

Kaman Chan

Yoram Barak

Ericka Bermudez

Melissa Ann Mayo

Chinping Chng

William Casey Hallows

Catherine M Cho

Moulay Hicham Alaoui Ismaili

Yu Zhu

Rachel Cathleen Botham

Adam P Silverman

Svetlana Balatskaya

Jessica Anna Hurtak

David William Homan

Anders Matthew Knight

Alberto Ortega Guerra

Jessica P Lao

Gregory Allan Cope

Claus M Krebber

Smita Shankar

Nicholas J Agard

Judy Victoria Antonio Viduya

Mikayla Jianghongxia Krawczyk

Kenneth W Mitchell

Patricia Choudhary

Trish Choudhary

Christopher Keltie Prier

The present invention provides engineered acid alpha-glucosidase (GAA) polypeptides and compositions thereof. In some embodiments, the engineered GAA polypeptides have been optimized to provide increased expression, stability at neutral pH, and activity in cell lysates. The invention also provides methods for utilization of the compositions comprising the engineered GAA polypeptides for therapeutic and other purposes.

Engineered acid alpha-glucosidase variants

The present invention provides engineered deoxyribose-phosphate aldolase polypeptides useful under industrial process conditions for the production of pharmaceutical and fine chemical compounds.

Engineered deoxyribose-phosphate aldolases

The present invention provides engineered glycosyltransferase (GT) enzymes, polypeptides having GT activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention provides engineered sucrose synthase (SuS) enzymes, polypeptides having SuS activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the GT enzymes and methods of using the engineered GT enzymes to make products with β-glucose linkages. The present invention further provides compositions and methods for the production of rebaudiosides (e.g., rebaudioside M, rebaudioside A, rebaudioside I, and rebaudioside D). The present invention also provides compositions comprising the SuS enzymes and methods of using them. Methods for producing GT and SuS enzymes are also provided.

Engineered glycosyltransferases and steviol glycoside glucosylation methods

The present invention provides engineered uridine phosphorylase (UP) enzymes, polypeptides having UP activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing UP enzymes are also provided. The present invention further provides compositions comprising the UP enzymes and methods of using the engineered UP enzymes. The present invention finds particular use in the production of pharmaceutical compounds.

Engineered uridine phosphorylase variant enzymes

The present invention provides engineered phosphopentomutase (PPM) enzymes, polypeptides having PPM activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing PPM enzymes are also provided. The present invention further provides compositions comprising the PPM enzymes and methods of using the engineered PPM enzymes. The present invention finds particular use in the production of pharmaceutical compounds.

Engineered phosphopentomutase variant enzymes

The present invention provides engineered proline hydroxylase polypeptides for the production of hydroxylated compounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hydroxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active pharmaceutical agents.

Biocatalysts and methods for hydroxylation of chemical compounds

The present invention provides engineered ligase polypeptides and compositions thereof, as well as polynucleotides encoding the engineered ligase polypeptides. The invention also provides methods for use of the compositions comprising the engineered ligase polypeptides for diagnostic and other purposes.

Engineered ligase variants

Engineered proline hydroxylase biocatalysts for hydroxylation of chemical compounds

The present invention provides engineered acetate kinase (AcK) enzymes, polypeptides having AcK activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing AcK enzymes are also provided. The present invention further provides compositions comprising the AcK enzymes and methods of using the engineered AcK enzymes. The present invention finds particular use in the production of pharmaceutical compounds.

Engineered acetate kinase variant enzymes

The present invention provides engineered DNA polymerase polypeptides and compositions thereof, as well as polynucleotides encoding the engineered DNA polymerase polypeptides. The invention also provides methods for use of the compositions comprising the engineered DNA polymerase polypeptides for diagnostic and other purposes.

Engineered DNA polymerase variants

The present invention provides engineered phenylalanine ammonia-lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia-lyase (PAL) polypeptides.

Engineered phenylalanine ammonia lyase polypeptides

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South San Francisco, CA, United States of America

Jonathan Vroom