Leland Stanford Junior University

California Institute of Technology

Jonathan Tsai

Irving L Weissman

David Baltimore

Alejandro Benjamin Balazs

Rahul Sinha

Yuval Rinkevich

Nathaniel Fernhoff

Aspects of the present disclosure include methods of treating a subject to reduce adhesion formation, the method comprising administering to a subject in need of thereof an agent that that targets adhesion-formation by injured mesothelial cells. The agent can act at a variety of checkpoints in the development of adhesions by injured mesothelial cells, including: targeting the injured mesothelial cells for destruction, recruiting inflammatory macrophages to the site of adhesion, preventing neutrophil recruitment to the site of adhesion, and/or inhibiting the expression or activity of a gene product whose expression is induced in the injured mesothelial cells. Compositions and kits for performing the methods are also provided.

Methods and compositions for the prevention and treatment of surgical adhesions

Disclosed herein are methods and materials for isolating and identifying T cell receptors from single cells. In some embodiments, genomic DNA from a single T cell is isolated using whole genome amplification (WGA). A series of PCR reactions is carried out to enrich the genomic template for sequences encoding the TCR alpha and beta chains, and then to isolate the sequences encoding the TCR alpha and beta chains.

Isolation of unknown rearranged T-cell receptors from single cells

Growing community of inventors

Saratoga, CA, United States of America

Jonathan Tsai