Exelixis, Inc.

Jonathan Scott Margolis

Helen Louise Francis-Lang

Bindu Priya Reddy

Andrew Roy Buchman

John William Winslow

David Andrew Ruddy

Darren Platt

Yuling Luo

Candace Swimmer

Casey Kopczynski

Sheila Akiko Homburger

Allen James Ebens, Jr

Jonathan Cagampang Heller

Catherine Sue Erickson

Christian Burks

Lucile A Gillett

The present invention relates to Drosophila genes and methods for their use. The invention provides nucleotide sequences of Drosophila genes, amino acid sequences of the encoded proteins, and derivatives (e.g., fragments) and analogs thereof. The invention further relates to fragments (and derivatives and analogs thereof) of proteins which comprise one or more domains of a Drosophila protein. Antibodies to Drosophila proteins, and derivatives and analogs thereof, are also provided. Also provided herein are vectors and host cells comprising such nucleic acids. Methods of production of a Drosophila protein (e.g., by recombinant means), and derivatives and analogs thereof, are provided. Chimeric polypeptide molecules comprising polypeptides of the invention fused to heterologous polypeptide sequences are provided. Methods to identify the biological function of a Drosophila gene are provided, including various methods for the functional modification (e.g., overexpression, underexpression, mutation, knock-out) of one gene, or of two or more genes simultaneously. Methods to identify a Drosophila gene which modifies the function of, and/or functions in a downstream pathway from, another gene are provided. The invention further provides for use of Drosophila proteins as media additives or pesticides.

Drosophila sequences

Tandem pore domain weak inward rectifying K (TWIK) channel nucleic acids and proteins that have been isolated from and Leptinotarsa are described. The TWIK channel nucleic acids and proteins can be used to genetically modify metazoan invertebrate organisms, such as insects, coelomates, and pseudocoelomates, or cultured cells, resulting in TWIK channel expression or mis-expression. The genetically modified organisms or cells can be used in screening assays to identify candidate compounds which are potential pesticidal agents or therapeutics that interact with TWIK channel proteins. They can also be used in methods for studying TWIK channel activity and identifying other genes that modulate the function of, or interact with, the TWIK channel gene.

Nucleic acids and polypeptides of invertebrate TWIK channels and methods of use

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San Francisco, CA, United States of America

Jonathan Scott Margolis