University of California

Massachusetts Institute of Technology

The Broad Institute, Inc.

Jonathan S Weissman

John Newman

Luke A Gilbert

Sina Ghaemmaghami

Nicholas Ingolia

Martin Kampmann

Aviv Regev

Eric Lander

Le Cong

Alexander K Shalek

Orit Rozenblatt-Rosen

Lei S Qi

Jellert Gaublomme

Thomas W Norman

Brian Cleary

Atray Dixit

Oren Parnas

James K Nunez

Onn Brandman

Calvin H Jan

Michael Bassik

Brittany Adamson

Lee Stirling Churchman

Jin Chen

Max Horlbeck

Provided herein are, inter alia, compositions and methods for manipulation of genomes of living organisms.

Compositions and methods for gene editing

Methods, compositions, and kits are provided for CRISPR/Cas mediated transcriptional modulation.

CRISPR/Cas transcriptional modulation

The present invention provides tools and methods for the systematic analysis of genetic interactions, including higher order interactions. The present invention provides tools and methods for combinatorial probing of cellular circuits, for dissecting cellular circuitry, for delineating molecular pathways, and/or for identifying relevant targets for therapeutics development.

Assays for massively combinatorial perturbation profiling and cellular circuit reconstruction

Provided herein are methods directed to multiplexed detection of the modulation of transcriptional activity using perturbation elements.

Pooled method for high throughput screening of trans factors affecting RNA levels

The present invention provides methods for conducting screens using nucleic acid elements (e.g., interfering RNAs) to confidently identify hit genetic elements. The present invention further comprises constructing vectors that contain two or more nucleic acid elements to knock down all pairwise combinations of the hit genetic elements identified from the screen. Following quantitation of the single and double-knockdown phenotypes, genetic interactions between all gene pairs can be calculated. Genes can then be clustered according to the similarity of the pattern of their interactions with all of the other genes to obtain a genetic interaction map, which can advantageously be used to predict functional associations between genes and identify drug targets for therapy such as combination cancer therapy.

Methods for genome-wide screening and construction of genetic interaction maps

Methods are provided for, inter alia, detecting nucleic acid molecules resistant to degradation, such as a plurality of RNA molecules bound to a ribosome, using various technologies including deep sequencing.

Methods for detecting modification resistant nucleic acids

Methods and compositions for identifying a cellular nascent RNA transcript are provided.

Methods of identifying a cellular nascent RNA transcript

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San Francisco, CA, United States of America

Jonathan S Weissman