Rensselaer Polytechnic Institute

University of Iowa Research Foundation

Other

University of California

Biotechnology Research and Development Corporation

Tate & Lyle Public Limited Company

USA As Represented by Secretary of the Navy

US Government As Represented by the Secretary of the Army

The Rockefeller University

Albany Molecular Research, Inc.

Massachusetts Institute of Technology

The United States Government As Represented by the Department of Veterans Affairs

Darpa

Nspc Technologies, LLC

Kabio R&d Soo Co., Ltd.

Jonathan S Dordick

Douglas S Clark

Robert J Linhardt

David Curtis Turner

Banahalli R Ratna

Paul E Schoen

Michael A Markowitz

Bruce P Gaber

Paul Roger Kust

Jungbae Kim

Riaz A Khan

Trevor John Simmons

Andrew J Hacking

Yuri L Khmelnitsky

Damodar R Patil

Daisuke Mori

Li Fu

Makoto Hirakane

Seok-Joon Kwon

David G Rethwisch

Kevin Li

Jeffrey M Friedman

David L Kaplan

Ferdinando F Bruno

Joseph A Akkara

Richard W Siegel

Peter C Michels

Sarah Stanley

Steven M Cramer

Aravind Srinivasan

Vikram M Paradkar

Brett D Martin

Maria Vladimir Sergeeva

Jeremy Sauer

Kaushal Rege

Fuming Zhang

Minoru Miyauchi

Scott Joel Novick

Ping Wang

Alexander M Klibanov

Thomas V Doherty

Sang-hyun Lee

David H Sherman

Peter S Cheetham

Shanghui Hu

Elner B Rathbone

Khizar S Mufti

Maria V Sergeeva

Ravindra S Kane

Alexander Ya Usyatinsky

Zhenyu Wang

Joseph O Rich

Jong Hwan Kwak

Ujjwal Bhaskar

Yuri Khmelnitsky

Domyoung Kim

Inseon Lee

Lakshmi Santhanam

Michael G Hogg

James A Holland

John M Arnold

Moo-Yeal Lee

Bosung Ku

Xi Qian

Sharon S Chen

Marc Douaisi

Jennifer E Gagner

Navdeep Grover

Payel Datta

Elena Paskaleva

Lei Lin

Prashanth Asuri

Paul Brodfuehrer

William J Sonstein

Sandeep S Karajanagi

Akihiro Onishi

Alexey A Vertegel

Xiaoqiu Wu

Jianjun Miao

Jong-Won Lee

Cheryl L Budde

Methods and systems are directed to multiplex detection of a bacterial pathogen in a sample. A first biotinylated lysin-derived cell wall binding domain is complexed with an avidin layer on a surface. A first bacterial pathogen detection complex including a second biotinylated lysin-derived cell wall binding domain, a detection domain, and an avidin linker complexed between the cell wall binding domain and the detection domain is also provided. The cell wall binding domains are derived from an endolysin, autolysin, bacteriocin, or exolysin, and are configured to bind a cell wall of a target bacterial pathogen. The detection domain includes one or more enzymes, fluorescent material, or DNA for emitting a signal for detection. Target bacterial pathogens present in a sample can thus be detected in a sandwich assay exhibiting increased selectivity and reduced limit of detection relative to traditional ELISA.

Multiplex detection of bacterial pathogens via cell wall binding domain complexes

The present invention provides methods and compositions for the remote control of cell function based on the use of a magnetic field to excite paramagnetic nanoparticles targeted to specific cell types. The cell type of interest expresses an ion channel wherein excitation of the paramagnetic nanoparticles results in a physical change that is transduced into a cellular response. Such cellular responses may include, for example, increases in gene expression resulting in production of one or more physiologically active proteins. The expression of such proteins can be used to treat a variety of different inherited or acquired diseases or disorders in a subject.

Compositions and methods to modulate cell activity

The present disclosure relates to synthesis of heparin, which may be bioequivalent to porcine USP Heparin Sodium. The synthesis may involve three intermediates starting from heparosan.

Biosynthetic heparin

A heparin structure with increased anticoagulant activity and method of making the same are disclosed. A heparin sample is provided and treated with a heparan sulfate sulfotransferase in an enzymatic reaction to add sulfuryl groups from a sulfuryl group source to the heparin sample, resulting in a heparin structure having above about 8% more 3-O-sulfo groups relative to wild-type bovine intestinal heparin. The added sulfuryl groups modify the heparin structure and increase the sample's binding to antithrombin III and its anticoagulant activity to be more similar and a viable alternative to porcine intestinal heparin. The modified heparin exhibits an anti-FXa activity and an anti-FIIa activity greater than about 180 U/mg, and a ratio of the anti-FXa activity to the anti-FIIa activity of about 0.9 to about 1.1, consistent with U.S. Pharmacopeia (USP) heparin activity specifications.

Enzymatic preparation of increased anticoagulant bovine sourced heparin

The invention encompasses methods and test strips for detecting the presence of cerebrospinal fluid (CSF) in a biological sample comprising removing sialo-transferrin and selectively detecting or measuring asialo-transferrin in the biological sample.

Detection of cerebrospinal fluid

Provided are a conjugate including a core and sialic acids or derivatives thereof bound to the surface of the core, and use thereof. The conjugate provided in the present invention binds with hemagglutinin on the surface of influenza virus to inhibit the course of infection of influenza virus, thereby preventing or treating infection of influenza virus and also preventing or treating infection of influenza virus resistant to antiviral agents. Accordingly, the conjugate may be widely used in the development of prophylactic or therapeutic agents for influenza virus infection.

Conjugate including core and sialic acid or derivative thereof bound to surface of core and use thereof

Provided is a gold nanocage with pores, charged ligand molecules covalently bound to internal surfaces of the gold nanocage, and payload molecules electrostatically adsorbed onto said charged ligand molecules, wherein a pore diameter is between 1.2 and 20 times a gyration radius of the payload molecule. Also provided is a method for making a nanoparticle, including using polyvinylpyrrolidone as a capping agent in a galvanic replacement reaction to convert a silver nanocube into a gold nanocage having pores, replacing the polyvinylpyrrolidone on internal surfaces with charged ligand molecules, and electrostatically adsorbing payload molecules onto the charged ligand molecules, with a pore diameter less than twenty times a gyration radius of the payload molecule. Also provided is a method of delivering a pharmacological agent to a mammalian cell, including contacting the cell with a gold nanocage having pores, ligand molecules bound to internal surfaces, and pharmacological agent adsorbed onto ligand molecules.

Internalization of proteins into hollowed gold nanostructures

This invention is directed to the application of a previously unknown property of nanomaterials&#x2014;its ability to enhance protein activity and stability at high temperatures, in organic solvents, and in polymer composites. Nanomaterials such as single-walled carbon nanotubes (SWNTs) can significantly enhance enzyme function and stability in strongly denaturing environments. Experimental results and theoretical analysis reveal that the enhancement in stability is a result of the curvature of these nanoscale materials, which suppresses unfavorable protein-protein interactions.

Enhanced stability of proteins immobilized on nanoparticles

Embodiments of the invention provide a cellulose-sheathed carbon nanotube fiber. One aspect of the invention provides a sheathed nanotube fiber comprising: a carbon nanotube fiber; and a cellulose sheath extending co-axially along at least a first portion of a length of the carbon nanotube fiber. Another aspect of the invention provides a method of forming a sheathed carbon nanotube fiber, the method comprising: co-electrospinning a carbon nanotube fiber gel core within a cellulose solution sheath.

Cellulose sheathed nanotube fiber

A method for the production of heparosan from fermentation culture ofK5 suitable for industrial production, exhibiting superior yield and purity, smaller culture volumes, faster growth, and lower costs.

K5 heparosan fermentation and purification

The invention is directed a synthetic wood composite comprising biomimetic macromolecules and methods for the preparation thereof.

Synthetic wood composite

A system and method for conducting high-throughput interactions between test compositions and analytes, comprising one or more test compositions, and a plurality of independent micromatrices, wherein each said micromatrix encapsulates at least one said test composition; and said micromatrices are made of a material that is permeable to an analyte.

Biocatalytic solgel microarrays

Aminoglycoside-polyamines are disclosed along with methods of use thereof in displacement chromatography and as DNA-binding ligands. The aminoglycoside-polyamines are derivatives of carbohydrates, such as sugars, amino sugars, deoxysugars, glycosides, nucleosides and their substituted counterparts. The subject polyamines possess a group in place of at least one hydrogen atom of at least one hydroxyl group of the carbohydrate compound. In these compounds Ris an alkyl group or an azaalkyl group, and Ris a primary or secondary amino group.

Aminoglycoside-polyamine displacers and methods of use in displacement chromatography

Growing community of inventors

Schenectady, NY, United States of America

Jonathan S Dordick