Medtronic Minimed, Inc.

Jonathan Reilly

David J Vachon

Glenn Noronha

Aaron M Heiss

Joseph C Walsh

Bill C Ponder

William P Van Antwerp

Brooks B Cochran

Fluorescent biosensor molecules, fluorescent biosensors and systems, as well as methods of making and using these biosensor molecules and systems are described. These biosensor molecules address the problem of obtaining fluorescence emission at wavelengths greater than about 500 nm. Biosensor molecules generally include an (1) an acridine-based fluorophore, (2) a linker moiety and (3) a boronate substrate recognition/binding moiety, which binds polyhydroxylate analytes, such as glucose. These biosensor molecules further include a 'switch' element that is drawn from the electronic interactions among these submolecular components. This fluorescent switch is generally 'off' in the absence of bound polyhydroxylate analyte and is generally &#x201c;on&#x201d; in the presence of bound polyhydroxylate analyte. Thus, the reversible binding of a polyhydroxylate analyte essentially turns the fluorescent switch &#x201c;on&#x201d; and &#x201c;off&#x201d;. This property of the biosensor molecules, as well as their ability to emit fluorescent light at greater than about 500 nm, renders these biosensor molecules particularly well-suited for detecting and measuring in-vivo glucose concentrations.

Analyte sensing via acridine-based boronate biosensors

Improved polymer matrices which incorporate fluorescent biosensor molecules as well as methods of making and using these polymer matrices are described. Such matrices can be used in fluorescent biosensors and biosensor systems, including those which are used in the detection of polyhydroxylated analytes such as glucose. The properties of the polymer matrices of the invention renders biosensors utilizing such matrices particularly well-suited for detecting and measuring in-vivo glucose concentrations.

Polymers functionalized with fluorescent boronate motifs and methods for making them

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Reseda, CA, United States of America

Jonathan Reilly