Inv Nylon Chemicals Americas, LLC

Jonathan Paul Combe

Alexander Brett Foster

Stephen Thomas Cartman

Arghya Barman

Paul S Pearlman

Gary J Smith

Jonathan Kennedy

Mark Paul Taylor

Andrew Shaw

Daniel Bawdon

Cristina Serrano Amatriain

Leonard Keith Pattenden

Massimiliano Zampini

Provided herein are methods for increasing the yield of an extracellular product synthesized by an organism cultured in a continuous aerobic fermentation system. The extracellular product yield is increased through the use of an organism modified to decreased production of polyhydroxyalkanoate, to increase production of the extracellular product, and to include promoters that can be inducible in response to nutrient limitation conditions. The extracellular product yield is also increased by operating the continuous fermentation system under particular nutrient limitation conditions. Also provided are non-naturally occurring organisms that have been modified for use with the provided methods, and extracellular products made using the provided methods.

Materials and methods for controlling limitation conditions in product biosynthesis for non-PHB generating species of the generaorand organisms related thereto

Methods for increasing carbon-based chemical product yield in an organism by perturbing redox balance in an organism as well as nonnaturally occurring organisms with perturbed redox balance and methods for their use in producing carbon-based chemical products are provided.

Materials and methods for controlling oxidation and reduction in biosynthetic pathways of species of the genera ralstonia and cupriavidus and organisms related thereto

Methods and materials for the production of hydroxy fatty acid anions, including 2-hydroxyisobutyric acid (2-HIBA), and/or derivatives thereof and compounds related thereto are provided. Also provided are products produced in accordance with these methods and materials.

Methods and materials for the biosynthesis of hydroxy fatty acid anions and/or derivatives thereof and/or compounds related thereto

Genetically engineered hosts and methods for their production and use in synthesizing hydrocarbons are provided.

Methods, materials, synthetic hosts and reagents for the biosynthesis of hydrocarbons and derivatives thereof

This disclosure relates to genome-scale attenuation or knockout strategies for directing carbon flux to certain carbon based building blocks within the 7-aminoheptanoic acid (7-AHA) and 6-aminohexanoic acid (6-AHA) biosynthesis pathways, for example, to achieve reduced flux to unwanted side products while achieving increased production of desired intermediates and end products. This disclosure also relates to non-naturally occurring mutant bacterial strains comprising one or more gene disruptions in aldehyde reductase and/or aldehyde dehydrogenase genes that are generated to direct carbon flux to certain carbon based building blocks. This disclosure further relates to a method for enhancing production of carbon based building blocks by generating non-naturally occurring mutant bacterial strains, culturing said mutant bacterial strains in the presence of suitable substrates or under desired growth conditions, and substantially purifying the desired end product.

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Redcar, United Kingdom

Jonathan Paul Combe