Pioneer Hi-bred International, Inc.

Other

Curagen Corporation

Jonathan P Duvick

Joyce R Maddox

Jacob T Gilliam

Xun Wang

Tracy A Rood

Oswald R Crasta

Otto Folkerts

Benjamin A Bowen

Aragula Gururaj Rao

Carl R Simmons

Guihua Lu

Steven P Briggs

Xu Hu

Dennis L Bidney

James J English

Pedro A Navarro Acevedo

Carol A Hendrick

Ranjini Chatterjee

Yogesh Kumar Sharma

Zhangmeng Bao

Mikhailo V Kolomiets

Maria Fedorova

New fumonisin detoxifying or fumonisin-derivative detoxifying homologues (both nucleic acids and proteins) are provided. Compositions which include these new proteins, recombinant cells, antibodies to the new homologues, and methods of using the homologues are also provided.

AP1 amine oxidase variants

The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated fromand. The polynucleotides may be mutated to remove glycosylation sites and cysteine residues. Additionally, the present invention provides recombinant expression cassettes, host cells, transgenic plants, and transgenic seed. The present invention also provides for polynucleotides containing both APAO and a fumonisin esterase. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and eukaryotic systems, methods for detecting fumonisins, and methods for identifying transformed plant cells. Methods for degrading fungal toxins in plants, grain, grain processing, silage, food crops and in animal feed are also disclosed.

Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use

The present invention provides transformed plants comprising a polynucleotide enconding amino polyol amine oxidase (APAO ) fromand a method of expressing APAO transgenic plants and seeds. In this way, transgenic plant can be produced having fumonisin degrading capability, as well as with the capability of producing the degrading enzymes.

Methods and nucleotide sequences encoding maize lipoxygenase proteins for modulating defense response are provided. The nucleotide sequences can be used in expression cassettes for modulating plant defense response. Transformed plants, plant cells and seed are also provided.

Lipoxygenase polynucleotides and methods of use

The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from . Additionally, the polynucleotide encoding for the APAO enzyme can be used to transform plant cells normally susceptible to or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. Additionally, the present invention provides for expressing both APAO and a fumonisin esterase in a transgenic plant. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and non-plant eukaryotic systems.

Amino polyol oxidase amine polynucleotides and related polypeptides and methods of use

The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from and . Additionally, the polynucleotides encoding for the APAO enzyme can be used to transform plant cells normally susceptible to Fusarium. Plants can be regenerated from the transformed plant cells.

Methods and compositions for modulating development and defense response are provided. Nucleotide sequences encoding a LOX protein are provided. Nucleotide sequences comprising the LOX promoter are also provided. The sequences can be used in expression cassettes for modulating development, developmental pathways, and the plant defense response. Transformed plants, plant cells, tissues, and seed are also provided.

Sunflower LOX polynucleotides and related compositions

Methods for identifying organisms capable of degrading fumonisin. Fumonisin can be incorporated into culture medium for selection of organisms resistant to fumonisin and/or capable of growing on fumonisin as a sole carbon source. Using this method, several organisms have been identified. These organisms can be used to isolate the enzymes and the genes responsible for conferring fumonisin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for fumonisin degrading enzymes can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.

Fumonisin detoxification compositions and methods

Compositions and methods to aid in protecting plants from invading pathogenic organisms are provided. The compositions of the invention comprise anti-pathogenic nucleotide sequences, including their promoters, and polypeptides encoded by the anti-pathogenic nucleic acid sequences. The compositions find use in methods for reducing or eliminating damage to plants caused by plant pathogens. Transformed plants, plant cells, tissues, and seed are also provided having enhanced disease resistance.

Sclerotinia-inducible promoters and their uses

The present invention provides methods and compositions relating to creating or enhancing disease resistance in plants. The invention provides isolated maize Rac nucleic acids and their encoded proteins that are involved in the altering the disease resistance pathway in plants, increasing transformation efficiency, inducing programmed cell death, and modulating the oxidative burst in a plant. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions.

Growing community of inventors

Des Moines, IA, United States of America

Jonathan P Duvick