Nanogen, Inc.

Nanogen/becton Dickinson Partnership

Gamida for Life B.v.

Jonathan M Diver

Jing Cheng

Michael James Heller

Lei Wu

James P O'Connell

Dan Smolko

Shila Jalali

David Willoughby

John J Carrino

Ed Sheldon

Louis O Gerrue

Edward L Sheldon

We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a 'laboratory-on-a-chip' system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

Integrated portable biological detection system

This invention relates to devices, methods, and compositions of matter for the multiplex amplification and analysis of nucleic acid sequences in a sample using ligation-dependent strand displacement amplification technologies in combination with bioelectronic microchip technology.

Multiplex amplification and separation of nucleic acid sequences using ligation-dependant strand displacement amplification an bioelectronic chip technology

We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a &ldquo;laboratory-on-a-chip&rdquo; system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

Multiplex amplification and separation of nucleic acid sequences using ligation-dependant strand displacement amplification and bioelectronic chip technology

Growing community of inventors

San Diego, CA, United States of America

Jonathan M Diver