Oy Alko Ab

Alko Ltd.

Alko-yhtiot Oy

Alko-yhtiot Oy (alko Group Ltd.)

Jonathan Knowles

Merja Elisa Penttila

Helena Nevalainen

Tuula Teeri

Paivi Lehtovaara-Helenius

Marja T Paloheimo

Pirkko Suominen

Arja Mantyla

Sirkka Keranen

Merja Pentilla

Sirpa Aho

Irma Salovuori

Helena M Nevalainen

Sunee Nitisinprasert

Functional derivatives of endoglucanase I (EGI), and the cloning and recombinant expression thereof, are described.

Yeast strains producing cellulolytic enzymes and methods and means for

Described are methods and means for the construction of strains of yeast capable of producing cellulolytic enzymes. This is achieved by the transfer of chromosomal genes or cDNA copies of mRNAs coding for cellulolytic enzymes isolated from the fungus Trichoderma reesei to yeast cells using recombinant DNA vectors capable of replicating in yeast. The correct expression of these cellulolytic genes in yeast leads to the production of cellulolytic enzymes which are secreted from the cell. This allows the yeast to hydrolyze 3-1,4-glucan substrates such as cellulose.

Method of making endoglucanase I

Methods and means for the construction of strains of yeast capable of producing cellulolytic enzymes, achieved by the transfer of chromosomal genes or cDNA copies of mRNAs coding for cellulolytic enzymes isolated from the fungus Trichoderma reesei to yeast cells using recombinant DNA vectors capable of replicating in yeast. The correct expression of these cellulolytic genes in yeast leads to the production of cellulolytic enzymes which are secreted from the cell. This allows the yeast to hydrolyze 3-1,4-glucan substrates such as cellulose.

DNA, vectors and transformed hosts encoding Trichoderma reesei

Enzyme preparations enriched in hemicellulase-, pectin-, and/or lignin-degrading enzymes are described which are also partially or completely deficient in cellulase degrading activity. Such preparations may be utilized in an crude, unpurified form and are especially useful in the production of pulp and paper.

Enzyme preparations with recombinantly-altered cellulose profiles and

Growing community of inventors

Helsinki, Finland

Jonathan Knowles