Danisco US Inc.

University of California

Jonathan K Lassila

Suzanne E Lantz

Bradley R Kelemen

Thijs Kaper

Slavko Kralj

Igor Nikolaev

Sandra W Ramer

Cheryl A Kerfeld

Sander Van Stigt Thans

Ronaldus Wilhelmus Hommes

Daniel Esteban Torres-Pazmino

Johannes Franciscus Thomas Van Lieshout

Markus Sutter

Maria Foukaraki

Wilhelmus Van Der Kley

Steven C Wilson

James N Kinney

Nicholai Douglas

Disclosed are compositions and methods relating to variant alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.

Alpha-amylase variants

To produce a bacterial microcompartment shell, or a designed shell based on naturally occurring bacterial microcompartment shells in a new host organism, a synthetic operon is constructed that contains the desired shell protein genes and translation efficiency is controlled by host specific ribosomal binding sites. Proteins or other molecules can be encapsulated in the microcompartment shells by various methods described herein. The constructs can also be used to express self-assembling sheets comprised of shell proteins.

Methods for producing microcompartments

Disclosed are glycosyl hydrolase enzyme variants, particularly variants of certain oxidoreductases of glycosyl hydrolase family 61. Nucleic acids encoding the glycosyl hydrolyase variants, compositions including the glycosyl hydrolase variants, methods of producing the variants, and methods of using the variants are also described.

Variant enzymes

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South San Francisco, CA, United States of America

Jonathan K Lassila