Quantumdx Group Limited

Jonathan James O'Halloran

Joseph H Hedley

Eduardo Boada

Heather Murton

Stephen Osborne

Jonathan Salmon

Thomas Michael Willshare

Hojjat Madadi

Chris Howell

Paul Marshall

Sam Whitehouse

Rachael Dixon

Sam Bhatt

John Tyson

Marc Flanagan

Emily Crozier

Philip Thomas Scully

Methods and devices for concentrating target cells using dielectrophoresis (DEP) are disclosed. The method allows relatively high throughput of sample through a microfluidic device in order to allow rapid capture of target cells even when they are present in low concentrations within the sample. The method utilizes multiple chambers through which samples will flow, the chambers arranged such that the first capture area has a larger area and faster flow rate than a second chamber, the second chamber being positioned downstream of the first capture area and being smaller with a slower flow rate to further concentrate the material captured in the first capture area.

Target cell concentration using dielectrophoresis (DEP)

A microfluidics device has one or more bubble diversion regions. Problems associated with the generation of air bubbles are avoided in a microfluidics device such as a cartridge, for use with a point of care (POC) diagnostics device, the cartridge being able to carry out downstream processing such as polymerase chain reaction (PCR) and/or nucleic acid capture. The bubble diversion region has a lower flow resistance than the flow resistance of an area of interest.

Microfluidic devices with bubble diversion

Embodiments of the present disclosure relate generally to reporter compositions which are synthetic nucleotides that comprise nucleotides with a high charge mass moiety attached thereto via a linker molecule. The linker molecules can vary in length in part to enable the high charge mass moiety to extend out from a DNA polymerase complex so that polymerization may not be influenced.

Design, synthesis and use of synthetic nucleotides comprising charge mass tags

A sorbent material is disclosed for the one-step separation of bio-macromolecules in a single pass extraction of DNA from complex mixtures of molecules and chemicals. In one embodiment, the sorbent material comprises a silanized material at least partially coated or formed with a polymer selected from the group consisting of a poly(aryl methacrylate), a poly(aryl acrylate), a poly(heteroaryl methacrylate, a poly(heteroaryl acrylate) and a copolymer thereof.

Sorbent material for separating bio-macromolecules

Various embodiments of the present disclosure generally relate to molecular biological protocols, equipment and reagents for the sequencing of target nucleic acid (DNA, RNA, cDNA, etc) molecules.

Methods and kits for nucleic acid sequencing

Embodiments of the present invention relate generally to strategies and methods of amplifying short target sequences and removing flanking sequences from target nucleic acids to remove background signal when detecting hybridizations events using sensitive detection biosensors, such as biosensors based on nanowires, carbon nanotubes, nanopores etc, that may be capable of detecting molecules at small molar concentrations (fM and less), or even at the single molecule level. Furthermore, by cropping and therefore standardizing the size of the target sequences to be detected, when detecting many target sequences in an array, the signals across each biosensor can be compared and the hybridization conditions standardized easily.

Sensing strategies and methods for nucleic acid detection using biosensors

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Newcastle upon Tyne, United Kingdom

Jonathan James O'Halloran