E.i. Dupont De Nemours and Company

Agrinomics LLC

Genus, Inc.

Agrigenetics, Inc.

Pioneer Hi-bred International, Inc.

Exelixis Plant Sciences, Inc.

Aagrinomics, LLC

Yadav, Narendra

Perez-grau, Luis

Kinney, Anthony John

Hitz, William Dean

Jonathan E Lightner

William Dean Hitz

John P Davies

Karen E Broglie

Stephanie Kay Clendennen

Anthony John Kinney

Narendra S Yadav

Andrew Mark Cigan

Matthew Campbell

Dylan Barnes

Brian Burger

John Joseph Okuley

Benjamin Beaton

William Thomas Christianson

Matthew Scott Culbertson

J Antoni Rafalski

Hein Tsoeng (Medard) Ng

Hein Tsoeng Ng

Luis Perez-Grau

Stephen M Allen

Mary Beatty

Nancy Anne Federspiel

James A Janni

Debra K Schuster

Jeremy E Coate

Steven Lorenz Wright

Omolayo O Famodu

Karlene H Butler

Emil M Orozco, Jr

D Ry Wagner

Theodore Mitchell Klein

Joan Tellefsen Odell

Leslie T Harvell

John D Everard

Jian-Ming Lee

Troy M Swartwood

Ry Wagner

Jill Van Winkle

Douglas O Keller

Luis Perez Grau

Federico Valverde

Natalie L Hubbard

Narendra Yadav

Antoni J Rafalski

The present disclosure relates to methods and compositions useful for prevention of porcine reproductive and respiratory syndrome virus (PRRSv) in animals, including animals of the species. The present teachings relate to swine wherein at least one allele of a CD163 gene has been inactivated, and to specific methods and nucleic acid sequences used in gene editing to inactivate the CD163 gene. Swine wherein both alleles of the CD163 gene are inactivated are resistant to porcine reproductive and respiratory syndrome virus (PRRSv). Elite lines comprising homozygous CD163-edited genes retain their superior properties.

Pig with a genetically modified CD163 gene resistant to PRRSv

Methods for improving the health of porcine species by targeted inactivation of CD163

The present disclosure relates methods and compositions useful for prevention of porcine reproductive and respiratory syndrome virus (PRRSv) in animals, including animals of the species. The present teachings relate to swine wherein at least one allele of a CD163 gene has been inactivated, and to specific methods and nucleic acid sequences used in gene editing to inactivate the CD163 gene. Swine wherein both alleles of the CD163 gene are inactivated are resistant to porcine reproductive and respiratory syndrome virus (PRRSv). Elite lines comprising homozygous CD163 edited genes retain their superior properties.

A novel method for prediction of the degree of heterotic phenotypes in plants is disclosed. Structural variation analyses of the genome are used to predict the degree of a heterotic phenotype in plants. In some examples, copy number variation is used to predict the degree of heterotic phenotype. In some methods copy number variation is detected using competitive genomic hybridization arrays. Further, methods for optimizing the arrays are disclosed, together with kits for producing such arrays, as well as hybrid plants selected for development based on the predicted results.

Using structural variation to analyze genomic differences for the prediction of heterosis

A novel method for prediction of the degree of heterotic phenotypes in plants is disclosed. Structural variation analyzes of the genome are used to predict the degree of a heterotic phenotype in plants. In some examples, copy number variation is used to predict the degree of heterotic phenotype. In some methods copy number variation is detected using competitive genomic hybridization arrays. Further, methods for optimizing the arrays are disclosed, together with kits for producing such arrays, as well as hybrid plants selected for development based on the predicted results.

Using oligonucleotide microarrays to analyze genomic differences for the prediction of heterosis

The present invention is directed to plants that display an altered oil content phenotype due to altered expression of a HIO32.2 nucleic acid. The invention is further directed to methods of generating plants with an altered oil content phenotype.

Generation of plants with altered oil content

The present invention is directed to plants that display an improved oil quantity phenotype or an improved meal quality phenotype due to altered expression of an HIO nucleic acid. The invention is further directed to methods of generating plants with an improved oil quantity phenotype or improved meal quality phenotype.

Generation of plants with altered oil, protein or fiber content

The present invention is directed to isolated HI041 nucleic acid and protein, and to plants that display an altered oil content phenotype due to altered expression of a HI041 nucleic acid. The invention is further directed to methods of generating plants with an altered oil content phenotype.

The present invention is directed to plants that display an altered oil content phenotype due to altered expression of a HIO102 nucleic acid. The invention is further directed to methods of generating plants with an altered oil content phenotype.

The present invention is directed to plants that display an altered oil content phenotype due to altered expression of a citrate synthase nucleic acid. The invention is further directed to methods of generating plants with an altered oil content phenotype.

The present invention is directed to an isolated starch, a transgenic plant or plant part producing the starch, flour and a thickened foodstuff prepared from a grain capable of producing such isolated starch. The present invention is directed to a method for altering starch amylose composition of a cereal grain.

Starches produced by the expression of heterologous granule bound starch synthase genes

The present invention is directed to plants that display an altered oil content phenotype due to altered expression of a HIO30 nucleic acid. The invention is further directed to methods of generating plants with an altered oil content phenotype.

The present invention is directed to plants that display an altered oil content phenotype due to altered expression of a HIO128.5 nucleic acid. The invention is further directed to methods of generating plants with an altered oil content phenotype.

The present invention is directed to plants that display an altered oil content phenotype due to altered expression of a HIO103.1 nucleic acid. The invention is further directed to methods of generating plants with an altered oil content phenotype.

Growing community of inventors

Des Moines, IA, United States of America

Jonathan E Lightner