Plant Bioscience Limited

Inari Agriculture Technology, Inc.

Jonathan Dallas George Jones

David Allen Jones

Michael Lee Nuccio

Michael Andreas Kock

Yajie Niu

John Patrick Casey, Jr

Randall William Shultz

Davide Sosso

Tomás Cermák

Maria Margarita D Unson

Jennifer A Raji

Richard Bailey Flavell

Eva Konecna

Cyril B Zipfel

Colwyn Martin Thomas

Mark Stewart Dixon

Peter John Balint-Kurti

Dagmar Renate Hann

John Paul Rathjen

The disclosure relates to precise modifications in wheat plant genomes including the heterologous integration of nucleic acid sequences that include a regulatory element at a predetermined locus. Also provided are wheat cells, wheat plants, wheat seeds, and processed wheat products comprising the modified wheat genomes.

Heterologous integration of regulatory elements to alter gene expression in wheat cells and wheat plants

Higher eukaryotes sense microbes through perception of pathogen-associated molecular patterns (PAMPs). The flagellin receptor FLS2 represents so far the only known pattern recognition receptor (PRR) inplants detect a variety of PAMPs including specific epitopes of the bacterial proteins flagellin and EF-Tu. Here, we show that flagellin and EF-Tu activate a common set of signalling events and defence responses, but without clear additive or synergistic effects. Treatment with either PAMP results in increased receptor sites for both PAMPs, a finding employed in a reverse-genetic approach to identify the receptor kinase EFR as the EF-Tu receptor. Transient expression of EFR inresults in formation of specific binding sites for EF-Tu, and responsiveness to this PAMP.efr mutants show a higher frequency of T-DNA transformation by the bacterium, revealing a role for EF-Tu perception in restricting this plant pathogen. These results demonstrate that EFR is the receptor for EF-Tu and that plant defence responses induced by PAMPs like EF-Tu reduce transformation by

Methods, means and compositions for enhancing agrobacterium-mediated plant cell transformation efficiency

The tomato Cf-2 gene has been cloned and its sequence provided, along with the encoded amino acid sequence. DNA encoding the polypeptide, and alleles, mutants and derivatives thereof, may be introduced into plant cells and the encoded polypeptide expressed, conferring pathogen resistance on plants comprising such cells and descendants thereof. The Cf-2 sequence comprises leucine rich repeats and the presence of such repeats enables identification of other plant pathogen resistance genes. Homologies to Cf-9 reveal motifs useful in the identification of other plant pathogen resistance genes.

Cf-2 plant pathogen resistance genes

The tomato Cf-4 gene has been isolated by positional cloning and its sequence provided, along with the encoded amino acid sequence. DNA encoding the polypeptide, alleles, mutants and derivatives thereof, and DNA encoding amino acid sequences showing a significant degree of homology thereto may be introduced into plant cells and the encoded polypeptide expressed, conferring pathogen resistance on plants comprising such cells and descendants thereof. The Cf-4 sequence shows a high degree of homology to Cf-9 and comprises leucine rich repeats.

Plant pathogen resistance genes and uses thereof

Growing community of inventors

Norwich, United Kingdom

Jonathan Dallas George Jones