Life Technologies Corporation

Invitrogen Corporation

Thermo Fisher Scientific Geneart Gmbh

Jonathan Chestnut

James L Hartley

Michael A Brasch

John J Carrino

Martin Anthony Gleeson

Gary F Temple

Knut R Madden

Louis Leong

David Cheo

Kevin G Hoff

James Fan

Matthias Arnold

Devon Rn Byrd

The invention generally relates to compositions and methods for designing and producing functional DNA binding effector molecules and associated customized services, tool kits and functional assays. In some aspects, the invention provides methods and tools for efficient assembly of customized TAL effector molecules. Furthermore, the invention relates to uses of TAL effector molecules and functional evaluation of such TAL by, for example, customized assays.

TAL-effector assembly platform, customized services, kits and assays

The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. The invention also provides host cells comprising nucleic acid molecules of the invention or prepared according to the methods of the invention, and also provides kits comprising the compositions, host cells and nucleic acid molecules of the invention, which may be used to synthesize nucleic acid molecules according to the methods of the invention.

Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites

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Carlsbad, CA, United States of America

Jonathan Chestnut