Monsanto Technology LLC

Jonathan C Lamb

Edward James Cargill

Richard J Lawrence

Artem G Evdokimov

Fenggao Dong

Thomas S Ream

David V Butruille

Paul S Chomet

Ty Jason Barten

Linda Rymarquis

Xuefeng Zhou

Dennis Hung Yang

Matthew Sean Marengo

Shiaw-Pyng Yang

Huachun Wang Larue

Charles L Armstrong

Ruth A Wagner

Bryce Lemke

Yang Ju Im

Brent A O'Brien

Fengxing Du

Daniel Challis

Brian W Gardunia

Piyaraj Nan Newton

Pierre Sehabiague

Shengqiang Zhong

Randall Kerstetter

Matthew J Bauer

Christopher T Bauer

Lisa Kanizay

Disclosed herein are plants exhibiting a semi-dwarf phenotype with reduced plant height compared to control wildtype plants. Some of the disclosed semi-dwarf plants comprise at least one non-natural brachytic mutation in which the activity of a BR2 gene is reduced. Also disclosed are methods for producing a semi-dwarf corn plant using a CRISPR based genome editing system.

Genome editing-based crop engineering and production of brachytic plants

This disclosure provides methods and compositions for increasing genome editing and site-directed integration events utilizing guided endonucleases and meiotic cell-preferred, egg cell-preferred or embryo tissue-preferred promoters.

Increasing gene editing and site-directed integration events utilizing meiotic and germline promoters

The present disclosure provides maize B chromosome genomic loci and methods of using for agronomic practices.

Recombinant maize B chromosome sequence and uses thereof

The invention provides novel methods to genotype haploid embryos using molecular assays. For example, quantitative methods for genotyping are provided. The methods provided also include providing a plurality of haploid kernels, determining the genotype of the haploid embryo of said kernels by distinguishing its genotype from the endosperm genotype, selecting a kernel having a desired genotype and producing doubled haploid plant from the selected kernel.

Methods for genotyping haploid embryos

Methods and compositions for improved plant breeding using gene editing and haploid induction are provided.

Methods and compositions for genome editing via haploid induction

The current invention provides methods for creating a block of genetically linked transgenic traits or a megalocus that can be transmitted as a single genetic unit. The present invention further provides methods for trait introgression to other plants, varieties or species using the megaloci of the invention. Also provided are plants, seeds, and plant parts comprising the megaloci.

Creation and transmission of megaloci

The invention relates to biotechnology and provides novel methods for sequence-specific or sequence-directed transcription activator-like effector recombinase-mediated integration of DNA sequences of interest into host genomes. The invention also provides methods of use for novel plant transformation vectors and expression cassettes, which include novel combinations of chimeric recombinases with plant expression and transformation elements. Methods for gene-targeting, DNA sequence removal, genome modification, and molecular breeding are also provided.

Methods for targeted transgene-integration using custom site-specific DNA recombinases

The invention relates to biotechnology and provides novel compositions and methods for sequence-specific or sequence-directed transcription activator-like effector recombinases. The invention also provides novel plant transformation vectors and expression cassettes, which include novel combinations of chimeric recombinases with plant expression and transformation elements. Methods for gene-targeting, DNA sequence removal, and molecular breeding are also provided.

Compositions and methods for custom site-specific DNA recombinases

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Wildwood, MO, United States of America

Jonathan C Lamb