10x Genomics, Inc.

Max-Delbrück-Centrum Für Molekulare Medizin In Der

Jonathan Alles

Marlon Stoeckius

Layla Katiraee

Christina Galonska

Caroline Julie Gallant

James Michael Chell

Felice Alessio Bava

Nikolaos Karaiskos

Nikolaus Rajewsky

Ivano Legnini

Salah Ayoub

The present disclosure provides a method for analyzing a polyribonucleotide, wherein the polyribonucleotide is an mRNA molecule with a poly(A) tail, said method comprising: (a) obtaining a labeled polyribonucleotide by GI tailing; followed by (b) providing a second molecule comprising a first primer recognition sequence followed by a sequence of C nucleotide residues linked to a sequence of T nucleotide residues; followed by (c) obtaining a complex of said labeled polyribonucleotide and said second molecule; followed by (d) obtaining an extended second molecule by (d1) extending the 3' end of the second molecule by synthesizing a sequence that is complementary to the labeled polyribonucleotide; followed by (d2) extending the 3′ end of the second molecule by adding at least 1 to 5 C nucleotide residues, followed by a second primer recognition sequence; said method providing information on entire polyribonucleotides, in particular mRNA isoforms and their respective poly(A) tails.

Full-length RNA sequencing

Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

Methods for spatial analysis using RNA-templated ligation

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Berlin, Germany

Jonathan Alles