United States of America, As Represented by the Secretary of Agriculture

Jonathan A Zerkowski

Richard D Ashby

Daniel Solaiman

Thomas A Foglia

Robert A Moreau

Nereus W Gunther

Yanhong Liu

NRRL B-30761 produces monorhamnolipids with predominantly 3-hydroxydodecenoyl-3-hydroxydecanoate (C-C) or 3-hydroxydodecanoyl-3-hydroxydecanoate (C-C) as the lipid moiety under static growth conditions. The cloning and sequencing of three genes and proteins involved in the biosynthesis of monorhamnose-lipid (RL) is described. Expression of two of these genes, i.e., rhlA and rhlB, together inNRRL B-30761 increases RL production by at least 10-fold. Also the generation of a recombinantNRRL B-30761 capable of synthesizing dirhamnose-lipid (RL) is described. Characterization of RL and RL produced by the recombinantNRRL B-30761 is also described.

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The isolation and characterization of a glucosyltransferase gene fromis disclosed. Use of the glucosyltransferase enzyme for the production of glucosylated sterol and hydroxyl-fatty acid substrates is also disclosed. This enzyme has broad-specificity and is useful for the production of sophorolipids both in-vivo and in-vitro.

Use of glucosyltransferase gene

A sophorolid produced by a method involving reacting a compound of formula I with a compound of formula II wherein

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Wayne, PA, United States of America

Jonathan A Zerkowski