The United States of America, as Represented by the Secretary, Department of Health and Human Services

P3s Corporation

Jonas M Winchell

Maureen Diaz

Bernard W Wolff

Alvaro J Benitez

Bernard J Wolff

Agnes Warner

Brianna Petrone

Kathleen Thurman

Stephanie L Mitchell

Methods for detecting presence of one or more ofspp.,spp.,andnucleic acids in a sample, such as a biological sample obtained from a subject, or an environmental sample, are provided. This disclosure also provides probes, primers, and kits for detecting one or more ofspp.,spp.,andin a sample.

Real-time PCR for the detection of pathogens

Methods for detecting presence of one or more ofspp.,spp.,, andnucleic acids in a sample, such as a biological sample obtained from a subject, or an environmental sample, are provided. This disclosure also provides probes, primers, and kits for detecting one or more ofspp.,spp.,, andin a sample.

Disclosed herein are methods and compositions (e.g., oligonucleotide primers) for isothermal amplification and detection ofnucleic acids in a sample. In some embodiments, the methods include contacting a sample with a set of LAMP primers specific for aCARDS toxin-encoding nucleic acid under conditions sufficient to produce annucleic acid amplification product and detecting the resultingamplification product. Kits including sets of LAMP primers for detection ofCARDS toxin nucleic acids are also provided herein.

Methods and compositions for isothermal amplification and detection of mycoplasma pneumoniae

Methods for detecting(such asspp.,serogroup 1,, and/or) are disclosed. A sample suspected of containing one or morenucleic acids is screened for the presence or absence of that nucleic acid. Determining whethernucleic acid is present in the sample can be accomplished by contacting the sample with detectably labeled probes capable of hybridizing to anucleic acid and detecting hybridization between the probes and nucleic acids in the sample. Detection of hybridization indicates that anucleic acid is present in the sample. Also disclosed are probes and primers for the detection of, and kits that contain the disclosed probes and/or primers.

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Disclosed herein are methods for detecting presence of one or more ofspp.,spp.,, andnucleic acids in a sample, such as a biological sample obtained from a subject, or an environmental sample. This disclosure also provides probes, primers, and kits for detecting one or more ofspp.,spp.,, andin a sample.

Methods for detecting, andspp. are disclosed. A sample suspected of containing a nucleic acid of one or more of, andspp. is screened for the presence or absence of that nucleic acid. Determining whether the, orspp. nucleic acid is present in the sample can be accomplished by detecting hybridization between aprobe (such as a CARDS toxin probe), aprobe (such as a ArgR probe), or aspp. probe (such as a SsrA probe) and a nucleic acid in a sample. Also disclosed are probes and primers for the detection of, andspp., and kits that contain the disclosed probes and/or primers.

Real time PCR assay for detection of bacterial respiratory pathogens

Methods of detecting, including differentiating between species ofand/or strains ofare disclosed, for example to detect and genotype ainfection. A sample suspected of containing a nucleic acid of a, is screened for the presence of that nucleic acid. The presence of thenucleic acid indicates the presence of thebacterium. Determining whether anucleic acid is present in a sample can be accomplished by detecting hybridization between aspecific primer, aspecific primer, and/or agenotype-specific primer and thenucleic acid containing sample. Thus, primers for the detection, species-specific and/or genotype-specific identification ofare disclosed. Kits that contain the disclosed primers also are disclosed.

Growing community of inventors

Lilburn, GA, United States of America

Jonas M Winchell