Neuronova Ab

Spatial Transcriptomics Ab and Illumina, Inc.

Illumina Corporation

Spatial Transcriptomics Ab

10x Genomics Sweden Ab

Jonas Frisén

Alex Aravanis

Joakim Lundeberg

Gordon M Cann

Leila Bazargan

Ann Marie Janson

Ming Zhao

Urban Lendahl

Kioumars Delfani

Patrik Ståhl

Clas Johansson

Stefan Momma

Diana Clarke

Patrik STÅHL

Fredrik SALMÉN

Sanja Vickovic

A method for spatially tagging nucleic acids of a biological specimen, including steps of (a) providing a solid support comprising different nucleic acid probes that are randomly located on the solid support, wherein the different nucleic acid probes each includes a barcode sequence that differs from the barcode sequence of other randomly located probes on the solid support; (b) performing a nucleic acid detection reaction on the solid support to locate the barcode sequences on the solid support; (c) contacting a biological specimen with the solid support that has the randomly located probes; (d) hybridizing the randomly located probes to target nucleic acids from portions of the biological specimen; and (e) modifying the randomly located probes that are hybridized to the target nucleic acids, thereby producing modified probes that include the barcode sequences and a target specific modification, thereby spatially tagging the nucleic acids of the biological specimen.

Spatially distinguished, multiplex nucleic acid analysis of biological specimens

The present invention relates to a method of determining whether a test compound is a proliferation-inducing or proliferation-inhibiting agent of ependymal neural stem cells. The method comprises: (a) contacting an ependymal neural stem cell with the test compound: and (b) determining the proliferation of the ependymal neural stem cell, wherein if the proliferation of the ependymal neural stem cell is increased in the presence of the test compound as compared to the absence of the test compound, the test compound is identified as a proliferation-inducing agent or wherein if the proliferation of the ependymal neural stem cell is decreased in the presence of the test compound as compared to the absence of the test compound, the test compound is identified as a proliferation-inhibiting agent.

Method of isolating ependymal neural stem cells

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Stockholm, Sweden

Jonas Frisén