Spatial Transcriptomics Ab

10x Genomics, Inc.

Illumina Corporation

10x Genomics Sweden Ab

Neuronova Ab

Spatial Transcriptions Ab

Jonas Frisen

Joakim Lundeberg

Patrik Stahl

Alex Aravanis

Gordon M Cann

Leila Bazargan

Michael Schnall-Levin

Michael Ybarra Lucero

Tarjei Sigurd Mikkelsen

Enric Llorens Bobadilla

Maja Marklund

Jeffrey Mold

Camilla Engblom

Qirong Lin

Kim Thrane

Fredrik Salmen

Urban Lendahl

Kioumars Delfani

Ming Zhao

Ann Marie Janson

Stefan Momma

Diana Clarke

Clas Johansson

Lilian Wikström

Harriet Rönnholm

Alex Mercer

Per Lindquist

Patrik Ståhl

Johan Häggblad

Olof Zachrisson

Amina Ossoinak

Göran Bertilsson

Rikard Erlandsson

Anders Haegerstrand

Jessica Heidrich

Nina Hellström

Katarina Jansson

Jarkko Kortesmaa

Hanna Lundh

Jacqueline McGuire

Karl Nyberg

Cesare Patrone

Fredrik SALMÉN

Patrik STÅHL

Hanna Främme

Kristina Hellström

The present disclosure relates to materials and methods for spatially analyzing nucleic acids that have been fragmented with a transposase enzyme, alone or in combination with other types of analytes.

Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample

Provided herein are methods, compositions, and kits for the detection of immune cell clonotypes and immune cell analytes within a biological sample.

Spatial transcriptomics for antigen-receptors

The present invention relates to methods and products for localized or spatial detection and/or analysis of RNA in a tissue sample or a portion thereof, comprising: (a) providing an object substrate on which at least one species of capture probe, comprising a capture domain, is directly or indirectly immobilized such that the probes are oriented to have a free 3' end to enable said probe to function as a reverse transcriptase (RT) primer; (b) contacting said substrate with a tissue sample and allowing RNA of the tissue sample to hybridise to the capture probes; (c) generating cDNA molecules from the captured RNA molecules using said capture probes as RT primers; (d) labelling the cDNA molecules generated in step (c), wherein said labelling step may be contemporaneous with, or subsequent to, said generating step; (e) detecting a signal from the labelled cDNA molecules; and optionally (f) imaging the tissue sample, wherein the tissue sample is imaged before or after step (c).

Methods and product for optimising localised or spatial detection of gene expression in a tissue sample

Localized detection of RNA in a tissue sample that includes cells is accomplished on an array. The array include a number of features on a substrate. Each feature includes a different capture probe immobilized such that the capture probe has a free 3' end. Each feature occupies a distinct position on the array and has an area of less than about 1 mm. Each capture probe is a nucleic acid molecule, which includes a positional domain including a nucleotide sequence unique to a particular feature, and a capture domain including a nucleotide sequence complementary to the RNA to be detected. The capture domain can be at a position 3&#x2032; of the positional domain.

Methods of detecting analytes

A method for spatially tagging nucleic acids of a biological specimen, including steps of (a) providing a solid support comprising different nucleic acid probes that are randomly located on the solid support, wherein the different nucleic acid probes each includes a barcode sequence that differs from the barcode sequence of other randomly located probes on the solid support; (b) performing a nucleic acid detection reaction on the solid support to locate the barcode sequences on the solid support; (c) contacting a biological specimen with the solid support that has the randomly located probes; (d) hybridizing the randomly located probes to target nucleic acids from portions of the biological specimen; and (e) modifying the randomly located probes that are hybridized to the target nucleic acids, thereby producing modified probes that include the barcode sequences and a target specific modification, thereby spatially tagging the nucleic acids of the biological specimen.

Spatially distinguished, multiplex nucleic acid analysis of biological specimens

Growing community of inventors

Stockholm, Sweden

Jonas Frisen