Life Technologies Corporation

Hyclone Laboratories, Inc.

Jon Q Coleman

Jeremy K Larsen

David S Rowley

Dana L Tangren

A filter assembly for separating microcarriers from a fluid medium includes a collapsible container bounding a sterile compartment adapted to hold a fluid. An inlet port is attached to the container through which fluid flows into the compartment. An outlet port is attached to the container through which fluid flows out of the compartment. A filter is disposed within the compartment, the filter dividing the compartment into an inlet chamber that is fluidly coupled with the inlet port and an outlet chamber that is fluidly coupled with the outlet port, the filter allowing a medium to pass therethrough but preventing microcarriers disposed in the medium from passing therethrough.

Filter systems for separating microcarriers from cell culture solutions

A method for separating microcarriers from a medium includes transferring a suspension that includes medium, microcarriers and cells into a compartment of a filter assembly. The filter assembly includes: a container having an interior surface bounding the compartment; and a filter having a tubular stem with an encircling sidewall extending between a first end and an opposing second end, the first end being secured to the interior surface of the container and the second end projecting into the compartment of the container, a plurality of openings extend through the sidewall of the stem that are large enough to allow the medium and cells to pass therethrough while preventing the microcarriers from passing therethrough. The medium and cells within the compartment are allowed to pass through the plurality of openings of the filter while the microcarriers are retained within the compartment of the container.

Growing community of inventors

Pleasant View, UT, United States of America

Jon Q Coleman