Washington State University

University System of Maryland

The University of Edinburgh

Jon Michael Oatley

Melissa Joan Oatley

Christopher Bruce Alexander Whitelaw

Simon Geoffrey Lillico

Bhanu Prakash Telugu

The present invention provides livestock animals and methods to create recipient animals for spermatogonial stem cell transplantation through modulation of the NANOS gene. In one embodiment genome editing issued to create animals with insertions or deletions (indels) that inactivate or otherwise modulate NANOS gene activity so that resulting males lack functional germ cells yet retain functional somatic cells, and females are fertile. These males can then be transplanted with donor spermatogonial stem cells and used for breeding.

NANOS knock-out that ablates germline cells

The present invention relates to the production and culture of undifferentiated spermatogonial stem cells that can be maintained long term and are feeder free. The resultant feeder-free populations can be used in any of a number of protocols including the generation of progeny bulls. The present invention includes novel methods required for the successful enrichment of bovine spermatogonial stem cells, novel cell lines and other components used for the same, as well as the resultant stem cell compositions.

Feeder-free method for culture of bovine and porcine spermatogonial stem cells

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Pullman, WA, United States of America

Jon Michael Oatley