Pacific Biosciences of California, Inc.

Applied Biosystems, LLC

Applera Corporation

Jon M Sorenson

Stephen Wilson Turner

Kevin Travers

John S Eid

Benjamin A Flusberg

Kenneth Mark Maxham

Cheryl Heiner

Patrick Marks

Jonas Korlach

Mark F Oldham

Linda G Lee

Sam L Woo

Richard T Reel

Jeremiah Hanes

David Cox

Congcong Ma

Benjamin G Schroeder

Dale R Webster

John Lyle

Lei Jia

Austin B Tomaney

Willy K Wiyatno

Andrey Kislyuk

Jessica Lee

Robin A Emig

Jeffrey Wegener

Tyson A Clark

Igor D Vilfan

Joseph Puglisi

Keith P Bjornson

Mathieu Foquet

Fred C Christians

Adrian Nielsen Fehr

Donald M Gray

Kai C Yung

Hans Callebaut

Eric Olivares

Sylvia H Fang

Chen-Shan Chin

Bidhan P Chaudhuri

Mark Chaisson

Puneet Suri

Ali Bashir

Robert Sebra

Yuandan Lou

Susan Tang

Jason Underwood

Thomas E Birkett

Salil Kumar

Benjamin B Jones

Catherine E Frantz

Various embodiments of the teachings relate to a system or method for sample preparation or analysis in biochemical or molecular biology procedures. The sample preparation can involve small volume processed in discrete portions or segments or slugs, herein referred to as discrete volumes. A molecular biology procedure can be nucleic acid analysis. Nucleic acid analysis can be an integrated DNA amplification/DNA sequencing procedure.

Apparatus, system, and method using immiscible-fluid-discrete-volumes

Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

Classification of nucleic acid templates

Methods, devices, and systems for performing intermittent detection during analytical reactions are provided. Such methods facilitate collection of reaction data from disparate reaction times. Further, such methods are useful for reducing photo-induced damage of one or more reactants in an illuminated analytical reaction at a given reaction time. In preferred embodiments, the reaction mixture is subjected to at least one illuminated and non-illuminated period and allowed to proceed such that the time in which the reaction mixture is illuminated is less than a photo-induced damage threshold period.

Intermittent detection during analytical reactions

Identifying organisms in a sample using sequencing kinetic signatures

Identification of nucleic acid template-linked barcodes comprising nucleic acid modifications

Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Methods are provided that include linking forward and reverse strands of a double stranded segment to form circular template molecules, and obtaining sequence data from the circular template molecule to identify modified bases, for example including the use of bisulfite treatment. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

Methods of sequencing and identifying the position of a modified base in a nucleic acid

Nucleic acid sequence analysis

Methods for identifying nucleic acid modifications

Growing community of inventors

Oakland, CA, United States of America

Jon M Sorenson