Applied Biosystems, LLC

Invitrogen Corporation

Ambion, Inc.

Jon Kemppainen

Gary J Latham

Richard Conrad

Brittan L Pasloske

Robert Setterquist

Xingwang Fang

Keqin Gregg

Scott K Davis

Bonnie Reus

Wan Ji

Kristen Taylor

The invention relates to methods of separating polynucleotides, such as DNA, RNA and PNA, from solutions containing polynucleotides by reversibly binding the polynucleotides to a solid surface, such as a magnetic microparticle.

Modified surfaces as solid supports for nucleic acid purification

Improved ball mill disruption techniques. In different embodiments, disrupting particles that are not substantially spherical are used. In other embodiments, roughened disrupting particles are used. In other embodiments, larger disrupting particles are used. In each instance, improved disruption can be achieved.

Biological sample disruption techniques

Compositions and method for making and using a synthetic bovine DNase I are disclosed. More particularly, the sbDNase I of the present invention is a versatile enzyme that cleaves DNA nonspecifically to release 5'-phosphorylated nucleotides. The sbDNase I molecules of the present invention find particular use in a wide range of molecular biology applications, including: degradation of contaminating DNA after RNA isolation; RNA clean-up prior to, or in conjunction with, RT-PCR after in vitro transcription; identification of protein binding sequences on DNA (DNase I footprinting); prevention of clumping when handling cultured cells; tissue dissociation and creation of fragmented DNA for in vitro recombination reactions.

Compositions and methods of using a synthetic Dnase I

The present disclosure relates to methods of DNA amplification with a first primer that has a random sequence of nucleotides at its 3&prime; end and a generic sequence 5&prime; of the random nucleotides, as well as a second primer with the generic sequence of the first primer. The disclosure further relates to a method of precipitating DNA on a solid medium to improve DNA amplification. In a preferred embodiment, the presently disclosed methods are used for high-throughput genotyping of DNA samples, such as bloodstains or trace amounts of DNA.

Method for rapid amplification of DNA

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Austin, TX, United States of America

Jon Kemppainen