Battelle Memorial Institute

National Technology & Engineering Solutions of Sandia, LLC

University of California

Iowa Corn Promotion Board

Dyadic International, Inc.

Natl Tech & Engineering Solutions of Sandia, LLC

Jon K Magnuson

Ziyu Dai

Joonhoon Kim

Shuang Deng

John Michael Gladden

Scott E Baker

Linda L Lasure

Jed J Lynn

Beth A Hofstad

Kyle R Pomraning

Morgann C Reilly

Blake A Simmons

Arkady Panteleimonovich Sinitsyn

Richard P Burlingame

Marion McKinley Bradford

Saori Amaike Campen

Jinxiang Zhang

Kenneth S Bruno

David E Culley

Marco A Baez-Vasquez

Linda L Lasre

Recombinantgenetically modified to increase expression of g8846, renamed herein as aconitic acid exporter (aexA), are provided, which in some examples are also genetically inactivated for an endogenous cis-aconitic acid decarboxylase (cadA) gene. Such recombinantproduce more aconitic acid as compared to native. Also provided are methods of using such recombinantto increase production of aconitic acid and other organic acids, such as citric acid, itaconic acid, and 3-hydroxypropionic acid (3-HP).

Aconitic acid exporter (aexA) increases organic acid production in aspergillus

Aconitic acid exporter (aexA) increases organic acid production in

Fungi that are genetically inactivated for the mstC gene (or a homolog thereof) are provided, which can also be genetically modified to increase production of heterologous proteins from a glucoamylase promoter. Methods of using these fungi, for example to degrade a biomass, are also provided.

Gene targets for improved enzyme production in fungi

The present invention provides for anhost cell comprising a gene of interest operatively linked to an ecm33 promoter of an ascomycete fungi, wherein the gene of interest is heterologous to the ecm33 promoter and/or to. In some embodiments, the gene of interest is a glycoside hydrolase enzyme. In some embodiments, the glycoside hydrolase enzyme is a glucosidase.

Promoter useful for high expression of a heterologous gene of interest in

This application provides recombinantfungi having an endogenous cis-aconitic acid decarboxylase (cadA) gene genetically inactivated, which allows aconitic acid production by the recombinant fungi. Such recombinant fungi can further include an exogenous nucleic acid molecule encoding aspartate decarboxylase (panD), an exogenous nucleic acid molecule encoding &#x3b2;-alanine-pyruvate aminotransferase (BAPAT), and an exogenous nucleic acid molecule encoding 3-hydroxypropionate dehydrogenase (HPDH). Kits including these fungi, and methods of using these fungi to produce aconitic acid and 3-hydroxypropionic acid (3-HP) are also provided.

Production of organic acids fromcis-aconitic acid decarboxylase (cadA) deletion strains

This disclosure provides-mediated transformation methods for the oil-producing (oleaginous) yeastsp., as well as yeast produced by the method. Such methods utilizesp. cells that have a T-DNA binary plasmid, wherein the T-DNA binary plasmid comprises a first nucleic acid molecule encoding a first protein and a second nucleic acid molecule encoding a selective marker that permits growth of transformedsp. cells in selective culture media comprising an antibiotic.

Agrobacterium-mediated transformation of lipomyces

The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.

Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus

Methods to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material are provided, as well as novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass.

Methods and compositions for degradation of lignocellulosic material

Growing community of inventors

Richland, WA, United States of America

Jon K Magnuson