Becton Dickinson and Company Limited

Jon E Salomon

Michael A Brasch

Alexander George Lastovich

Richard L Moore

Song Shi

Adrien P Malick

William Kevin Carpenter

John D Mantlo

Kristin Weidemaier

Joshua Herr

Owen Lewis Joyce

Susan M Kircher

Qihua Xu

William B Brasso

Eric Fallows

Liping Feng

David S Sebba

Vanda White

Richard Abbot

Cristian Clavijo

Xiao Mo

Tuan-Linh Ngoc Nguyen

Alexander Adam Papp

Sheryl Douglas-Mckay

Shirley Ng

Sean Connell

Meghan Wolfgang

Susan Kircher

A microbe-specific medium, containing specific chromogenic substrates, for the detection of vancomycin-resistant enterococci in a biological sample, whereby both the detection and identification of vancomycin-resistant enterococci at the species level is achieved utilizing one sample and one test.

Chromogenic medium for the detection and identification of vancomycin resistant enterococci and method therefor

Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.

Identification of microorganisms using MALDI-TOF-MS on-plate extraction

Chromogenic medium for the detection and identification of Vancomycin resistant enterococci and method therefor

Growing community of inventors

Stewartstown, PA, United States of America

Jon E Salomon